In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde. These microparticles were subsequently used to perform in-line capillary electrophoresis with clozapine as a model substrate. This setup could be used further to establish CE-IMERs of other drug metabolic enzymes in a commercially available liquid based capillary coolant system. The CE-IMER setup was successful, although a subsequent decrease in enzyme activity was observed on repeated runs.

• MeSH
• aminy chemie   MeSH
• design vybavení přístrojové vybavení   MeSH
• elektroforéza kapilární přístrojové vybavení   MeSH
• enzymy imobilizované chemie   MeSH
• glutaraldehyd chemie   MeSH
• klozapin chemie   MeSH
• lidé MeSH
• magnetické pole MeSH
• magnety chemie   MeSH
• mikrosféry * MeSH
• NADP chemie   MeSH
• oxid křemičitý chemie   MeSH
• oxygenasy chemie   MeSH
• povrchové vlastnosti MeSH
• sérový albumin hovězí chemie   MeSH
• stabilita enzymů MeSH
• teplota MeSH
• velikost částic MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH