The abc1(-)/coq8(-) gene deletion respiratory-deficient mutant NBp17 of fission yeast Schizosaccharomyces pombe displayed a phenotypic fermentation pattern with enhanced production of glycerol and acetate, and also possessed oxidative stress-sensitive phenotypes to H(2)O(2), menadione, tBuOOH, Cd(2+), and chromate in comparison with its parental respiratory-competent strain HNT. As a consequence of internal stress-inducing mutation, adaptation processes to restore the redox homeostasis of mutant NBp17 cells were detected in minimal glucose medium. Mutant NBp17 produced significantly increased amounts of O(2)•- and H(2)O(2) as a result of the decreased internal glutathione concentration and the only slightly increased glutathione reductase activity. The Cr(VI) reduction capacity and hence the •OH production ability were decreased. The mutant cells demonstrated increased specific activities of superoxide dismutases and glutathione reductase (but not catalase) to detoxify at least partially the overproduction of reactive oxygen species. All these features may be explained by the decreased redox capacity of the mutant cells. Most notably, mutant NBp17 hyperaccumulated yellow CdS.

• MeSH
• ABC transportéry genetika   MeSH
• delece genu MeSH
• fenotyp MeSH
• glutathion metabolismus   MeSH
• kadmium metabolismus   MeSH
• mikrobiální testy citlivosti MeSH
• oxidace-redukce MeSH
• oxidancia farmakologie   MeSH
• peroxidy metabolismus   MeSH
• Schizosaccharomyces pombe - proteiny genetika   MeSH
• Schizosaccharomyces účinky léků   genetika   metabolismus   MeSH
• ubichinon genetika   MeSH
• Publikační typ
• časopisecké články MeSH