A free living freshwater Pseudomonas strain ML96 was isolated from the Huguangyan Maar Lake in southern China. Genome sequencing of strain ML96 revealed a 4.7 Mb long draft genome consisting of 47 contigs with a G+C content of 64.8%. Its 16S and 23S rRNA gene sequences were 99.8% and 99.3% identical to those of its closest relative, Pseudomonas alcaligenes NBRC 14159, respectively. ML96's genome shared 73% orthologous CDS (3256/4457) with the genome of NBRC 14159. Comparative genomics analysis provide further insight into the diversity and evolution of aquatic Pseudomonas species, which may help enhance our understanding of this both environmentally and medically important group of bacteria.
- MeSH
- fylogeneze MeSH
- genom bakteriální MeSH
- jezera mikrobiologie MeSH
- mikrobiologie vody * MeSH
- molekulární sekvence - údaje MeSH
- Pseudomonas klasifikace genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Čína MeSH
The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, however, contained negligible lipid reserves (1-10% of DW). Treatments inducing hyperproduction of storage lipids in P. kessleri biomass were described. The cultures were grown in the absence or fivefold decreased concentration of either nitrogen or phosphorus or sulfur. Limitation by all elements using fivefold or 10-fold diluted mineral medium was also tested. Limitation with any macroelement (nitrogen, sulfur, or phosphorus) led to an increase in the amount of lipids; nitrogen limitation was the most effective. Diluted nutrient media (5- or 10-fold) were identified as the best method to stimulate lipid overproduction (60% of DW). The strategy for lipid overproduction consists of the fast growth of P. kessleri culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW. Cultivation conditions necessary for maximizing lipid content in P. kessleri biomass generated in a scale-up solar open thin-layer photobioreactor were described.
- MeSH
- biomasa MeSH
- biotechnologie MeSH
- chlorofyl analýza metabolismus MeSH
- Chlorophyta metabolismus MeSH
- fotobioreaktory MeSH
- kultivační média MeSH
- lipidy biosyntéza MeSH
- mastné kyseliny analýza metabolismus MeSH
- metabolismus lipidů MeSH
- mikrořasy metabolismus MeSH
- oxid uhličitý metabolismus MeSH
- škrob analýza metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
