1. vydání 164 stran : ilustrace, schémata, grafy ; 24 cm
Publikace se zaměřuje na elektrochemickou a biochemickou analýzu molekul. Určeno odborné veřejnosti.
- MeSH
- elektrochemické techniky MeSH
- elektrochemie MeSH
- klinické chemické testy MeSH
- molekulární biologie MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biochemie
- chemie, klinická chemie
Electrochemistry of nucleic acid is at present a booming field producing about 800 papers published per year. First papers in this field were published in 1958–1961 in Brno (Czech Republic) showing that purine and pyrimidine base residues in single-stranded DNA and RNA were reduced at Hg electrodes and the guanine residue produced an anodic signal when cyclic modes were used. The reduction sites of the base residues in native double-stranded (ds) DNA are hidden in the interior of the dsDNA molecule, which made their reduction difficult. At that time oscillographic polarography (ac chronopotentiometry) showed excellent sensitivity to changes in DNA structure and allowed to investigate DNA denaturation and hybridization. Later on also other electrochemical methods and electrodes were applied. In the following three decades basic principles were found which are at present used in the development of DNA hybridization sensors.
- MeSH
- chromozomy chemie MeSH
- dějiny 20. století MeSH
- dějiny 21. století MeSH
- DNA * analýza chemie MeSH
- elektrochemické techniky dějiny metody MeSH
- elektrochemie * dějiny metody MeSH
- plazmidy analýza chemie MeSH
- polarografie dějiny metody MeSH
- potenciometrie dějiny metody MeSH
- RNA transferová analýza chemie MeSH
- RNA virová analýza chemie MeSH
- Check Tag
- dějiny 20. století MeSH
- dějiny 21. století MeSH
- Publikační typ
- historické články MeSH
- práce podpořená grantem MeSH
Parkinson's disease (PD) is associated with the formation and deposition of amyloid fibrils of the protein alpha-synuclein (AS). It has been proposed that oligomeric intermediates on the pathway to fibrilization rather than the fibrils themselves are the pathogenic agents of PD, but efficient methods for their detection are lacking. We have studied the interfacial properties of wild-type AS and the course of its aggregation in vitro using electrochemical analysis and dynamic light scattering. The oxidation signals of tyrosine residues of AS at carbon electrodes and the ability of fibrils to adsorb and catalyze hydrogen evolution at hanging mercury drop electrodes (HMDEs) decreased during incubation. HMDEs were particularly sensitive to pre-aggregation changes in AS. Already after 1 h of a standard aggregation assay in vitro (stirring at 37 degrees C), the electrocatalytic peak H increased greatly and shifted to less negative potentials. Between 3 and 9 h of incubation, an interval during which dynamic light scattering indicated AS oligomerization, peak H diminished and shifted to more negative potentials, and AS adsorbability decreased. We tentatively attribute the very early changes in the interfacial behavior of the protein after the first few hours of incubation to protein destabilization with disruption of long-range interactions. The subsequent changes can be related to the onset of oligomerization. Our results demonstrate the utility of electrochemical methods as new and simple tools for the investigation of amyloid formation.
