Hydrophilic interaction liquid chromatography (HILIC) method using internal standard for the determination and stability study of ascorbic acid was developed. HILIC method was very fast and simple using the following analytical conditions: ZIC HILIC (150 x 2.1 mm, 3.5 microm) chromatographic column and mobile phase composed of ACN and 50 mM ammonium acetate buffer pH 6.8 (78:22 v/v). Diode array detection was performed and chromatograms were processed at 268 nm, the maximum wavelength of absorbance of ascorbic acid. An extensive stability study of ascorbic acid as a function of various factors including temperature, stabilizing agents, oxygen presence and its concentration in solution was performed in order to gain information about the quantitative influence of individual stability factors. Low temperature and stabilizing agents (o-phosphoric acid and oxalic acid) were found to be key factors enabling substantial enhancement of the stability of ascorbic acid.
- MeSH
- chromatografie kapalinová metody normy MeSH
- financování organizované MeSH
- indikátory a reagencie MeSH
- koncentrace vodíkových iontů MeSH
- kyselina askorbová analýza metabolismus normy MeSH
- kyselina chlorogenová analýza normy MeSH
- kyslík MeSH
- lidé MeSH
- referenční standardy MeSH
- rozpouštědla MeSH
- spektrofotometrie ultrafialová MeSH
- stabilita léku MeSH
- teplota MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- změna skupenství MeSH
- Check Tag
- lidé MeSH
