New waterbodies have been created in a postmining area of the brown-coal basin in Sokolov, Czech Republic. The former open-cast quarry, Medard, has been filling with water from a local river, the surrounding catchment spoil heaps, and acid mine drainages. The effect of acidic (pH down to 2.5) and high-conductivity water (up to 1400 mS/m) on selected cyanobacteria and the possibility of cyanobacterial water bloom in the newly formed Lake Medard were studied by means of chlorophyll fluorometry (actual photosystem II [PSII] quantum yield, ΔF/Fm', and relative electron transport rate, rETR). The acidic spoil-heap waters caused a decrease in cyanobacterial photosynthetic activity of 52 to 100% of the initial ΔF/Fm' value. The Dolichospermum strains were about 10 times more sensitive than Microcystis viridis. The high concentration of dissolved ions appeared to have less effect on cyanobacterial PSII. Although the bottom meta- and hypolimnion layers were proven to negatively influence the cyanobacteria, the perennial stratification of the lake does not enable the water characteristics of the upper layers to change extensively and thus possibly suppresses the undesirable cyanobacterial bloom. The response of cyanobacteria to spoil-heap waters appears to be species-specific and can promote selection of those resistant to postmining environments.
- MeSH
- chemické látky znečišťující vodu analýza toxicita MeSH
- druhová specificita MeSH
- fotosyntéza účinky léků MeSH
- monitorování životního prostředí metody MeSH
- sinice klasifikace účinky léků MeSH
- sladká voda mikrobiologie MeSH
- těžba uhlí * MeSH
- vodní zdroje analýza normy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Abundant phytoplankton and bacteria were identified by high-throughput 16S rRNA tag Illumina sequencing of samples from water and ice phases collected during winter at commercial fish ponds and a sand pit lake within the UNESCO Třeboň Basin Biosphere Reserve, Czech Republic. Bacterial reads were dominated by Proteobacteria and Bacteroidetes. Despite dominance by members of just two phyla, UniFrac principal coordinates analysis of the bacterial community separated the water community of Klec fish pond, as well as the ice-associated community of Klec-Sand Pit from other samples. Both phytoplankton and cyanobacteria were represented with hundreds of sequence reads per sample, a finding corroborated by microscopy. In particular, ice from Klec-Sand Pit contained high contributions from photoautotrophs accounting for 25% of total reads with reads dominated by single operational taxonomic units (OTUs) of the cyanobacterium Planktothrix sp. and two filamentous diatoms. Dominant OTUs recovered from ice were largely absent (< 0.01%) from underlying water suggestive of low floristic similarity of phytoplankton partitioned between these phases. Photosynthetic characterization of phototrophs resident in water and ice analysed by variable chlorophyll a fluorescence showed that communities from both phases were photosynthetically active, thus supporting ice as viable habitat for phytoplankton in freshwater lakes and reservoirs.
- MeSH
- Bacteria klasifikace genetika metabolismus MeSH
- biodiverzita MeSH
- ekosystém MeSH
- fotosyntéza MeSH
- fytoplankton klasifikace genetika metabolismus MeSH
- led * MeSH
- mikrobiologie vody * MeSH
- roční období MeSH
- rybníky MeSH
- sladká voda mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Česká republika MeSH
