Human induced pluripotent stem cell (iPSC) lines were generated from peripheral blood mononuclear cells (PBMCs) isolated from a patient diagnosed with spontaneous late-onset Alzheimer's disease (AD) carrying ApoE3/3 gene and one age-, sex-, and ApoE-matched healthy control. Reprogramming was done using a commercially available Epi5 Reprogramming Kit containing OCT4, SOX2, KLF4, LIN28, and L-MYC as reprogramming factors. The pluripotency of the iPSC lines was verified by the expression of pluripotency markers and by their capacity to differentiate into all three embryonic germ layers in vitro. These newly established iPSC lines offer a valuable platform for in vitro modeling of AD.

• MeSH
• Alzheimerova nemoc * genetika   metabolismus   MeSH
• apolipoprotein E3 genetika   MeSH
• buněčná diferenciace MeSH
• genotyp MeSH
• indukované pluripotentní kmenové buňky * metabolismus   MeSH
• Krüppel-like faktor 4 MeSH
• leukocyty mononukleární metabolismus   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH