Effects of processing and extraction solvents on antioxidant properties and other characteristics were evaluated for ten medicinal plant species originating from two different localities and two production years. A combination of spectroscopic and liquid chromatography techniques possessed data for multivariate statistics. Water, 50% (v/v) ethanol, and dimethyl sulfoxide (DMSO) were compared to select the most suitable solvent for the isolation of functional components from the frozen/dried medicinal plants. DMSO and 50% (v/v) ethanol were evaluated as more efficient for phenolic compounds and colorants extraction, while water was more useful for element extraction. Drying and extraction of herbs with 50% (v/v) ethanol was the most appropriate treatment to ensure a high yield of most compounds. The satisfactory differentiation of herbs (61.8-100%) confirmed the significant effect of the processing, geographical, and seasonal factors on target functional component concentrations. Total phenolic and total flavonoid compounds content, total antioxidant activity expressed as TAA, yellowness, chroma, and browning index were identified as the most important markers for medicinal plant differentiation.

• MeSH
• antioxidancia chemie   MeSH
• chromatografie kapalinová MeSH
• dimethylsulfoxid MeSH
• ethanol chemie   MeSH
• fenoly chemie   MeSH
• léčivé rostliny * chemie   MeSH
• multivariační analýza MeSH
• roční období MeSH
• rostlinné extrakty chemie   MeSH
• rozpouštědla chemie   MeSH
• voda MeSH
• Publikační typ
• časopisecké články MeSH

Effects of different factors on the content of phenolics, anthocyanins, antioxidants and colour parameters in two varieties of Aronia and three varieties of Saskatoon berries were evaluated. The berries were extracted by dynamic solvent extraction using three different solvents (water, 50% ethanol (v/v) and dimethyl sulfoxide) and subsequently analysed by ultraviolet visible near infrared spectroscopy and electron paramagnetic resonance followed by chemometric analysis. The results obtained show that, dimethyl sulfoxide was the most appropriate for berries functional components isolation, and among the studied berries, Aronia exhibited the highest contents of phenolics. Besides that, varieties of Aronia melanocarpa Viking and of Amelanchier lamarckii Ballerina were selected as the cultivars with high contents of functional components. Satisfactory differentiation of berries was achieved according to extraction solvent, fruit type, variety and production years. The results obtained reveal that, phytochemical composition of Aronia and Saskatoon berries may vary depending on genotypic factors, climate, growing conditions as well as on extraction method used for their assessment. Sample processing, genotypic and seasonal factors had significant effect on the concentration of naturally occurring compounds in berries.

• MeSH
• anthokyaniny MeSH
• antioxidancia MeSH
• fenoly MeSH
• ovoce MeSH
• Photinia * MeSH
• Rosaceae * MeSH
• rostlinné extrakty MeSH
• Publikační typ
• časopisecké články MeSH