polo and CDC5 are two genes required for passage through mitosis in Drosophila melanogaster and Saccharomyces cerevisiae, respectively. Both genes encode structurally related protein kinases that have been implicated in regulating the function of the mitotic spindle. Here, we report the characterization of a human protein kinase that displays extensive sequence similarity to Drosophila polo and S. cerevisiae Cdc5; we refer to this kinase as Plk1 (for polo-like kinase 1). The largest open reading frame of the Plk1 cDNA encodes a protein of 68,254 daltons, and a protein of this size is detected by immunoblotting of HeLa cell extracts with monoclonal antibodies raised against the C-terminal part of Plk1 expressed in Escherichia coli. Northern blot analysis of RNA isolated from human cells and mouse tissues shows that a single Plk1 mRNA of 2.3 kb is highly expressed in tissues with a high mitotic index, consistent with a possible function of Plk1 in cell proliferation. The Plk1 gene maps to position p12 on chromosome 16, a locus for which no associations with neoplastic malignancies are known. The Plk1 protein levels and its distribution change during the cell cycle, in a manner consistent with a role of Plk1 in mitosis. Thus, like Drosophila polo and S. cerevisiae Cdc5, human Plk1 is likely to function in cell cycle progression.

• MeSH
• buněčný cyklus MeSH
• DNA vazebné proteiny genetika   MeSH
• Drosophila melanogaster genetika   MeSH
• fungální proteiny genetika   MeSH
• genová knihovna MeSH
• geny MeSH
• HeLa buňky MeSH
• karcinom genetika   MeSH
• klonování DNA MeSH
• lidé MeSH
• lidské chromozomy, pár 16 * MeSH
• mapování chromozomů MeSH
• mitóza * MeSH
• molekulární sekvence - údaje MeSH
• monoklonální protilátky imunologie   MeSH
• nádory nosohltanu genetika   MeSH
• otevřené čtecí rámce MeSH
• protein-serin-threoninkinasy genetika   MeSH
• proteinkinasy genetika   imunologie   izolace a purifikace   fyziologie   MeSH
• proteiny buněčného cyklu * MeSH
• proteiny Drosophily * MeSH
• proteiny vázající RNA MeSH
• protoonkogenní proteiny MeSH
• regulace genové exprese u nádorů MeSH
• Saccharomyces cerevisiae - proteiny MeSH
• Saccharomyces cerevisiae genetika   MeSH
• sekvence aminokyselin MeSH
• sekvence nukleotidů MeSH
• sekvenční homologie aminokyselin MeSH
• sekvenční seřazení MeSH
• subcelulární frakce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH