Cervical lymphadenopathy in children usually has an infectious or reactive etiology, but patients with persistent findings, adolescent age and accompanying systemic symptoms or abnormal laboratory findings should undergo an early biopsy. In our fifteen-year-old girl, who observed cervical lymphomegaly for 4 weeks without improvement, Staphylococcus saprophyticus was cultured from a peroperative biopsy. Coagulase-negative staphylococci (CoNS) represent the main part of the microflora of the skin and mucous membranes of humans, but they can also be used as important opportunistic pathogens.
- MeSH
- Anti-Bacterial Agents administration & dosage therapeutic use MeSH
- Suppuration MeSH
- Coagulase MeSH
- Neck * surgery pathology MeSH
- Humans MeSH
- Lymphadenitis * surgery diagnosis therapy MeSH
- Adolescent MeSH
- Staphylococcal Infections diagnosis drug therapy MeSH
- Staphylococcus saprophyticus isolation & purification MeSH
- Check Tag
- Humans MeSH
- Adolescent MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
Chronic wounds and their associated bacterial infections are major issues in modern health care systems. Therefore, antimicrobial resistance (AMR), treatment costs, and number of disability-adjusted life-years have gained more interest. Recently, photodynamic therapy emerged as an effective approach against resistant and naïve bacterial strains with a low probability of creating AMR. In this study, needleless electrospinning was used to produce an indocyanine green (ICG) loaded poly(d,l-lactide) nanofibrous mesh as a photoresponsive wound dressing. The non-woven mesh had a homogeneous nanofibrous structure and showed long-term hydrolytic stability at different pH values. The antimicrobial activity was tested against several bacterial strains, namely Staphylococcus saprophyticus subsp. bovis, Escherichia coli DH5 alpha, and Staphylococcus aureus subsp. aureus. Upon irradiation with a laser of a specific wavelength (λ = 810 nm), the bacterial viability was significantly reduced by 99.978% (3.66 log10), 99.699% (2.52 log10), and 99.977% (3.64 log10), respectively. The nanofibrous mesh showed good biocompatibility, which was confirmed by the proliferation of mouse fibroblasts (L929) on the surface and into deeper parts of the mesh. Furthermore, a favorable proangiogenic effect was observed in ovo using the chorioallantoic membrane assay. In general, it can be concluded that ICG loaded nanofibers as an innovative wound dressing represent a promising strategy against chronic wounds associated with skin infections.
- MeSH
- Biocompatible Materials chemistry pharmacology MeSH
- Cell Line MeSH
- Chorioallantoic Membrane blood supply drug effects MeSH
- Escherichia coli drug effects growth & development MeSH
- Fibroblasts cytology drug effects MeSH
- Photochemotherapy MeSH
- Indocyanine Green chemistry pharmacology MeSH
- Microbial Viability drug effects MeSH
- Mice MeSH
- Nanofibers MeSH
- Bandages MeSH
- Polyesters chemistry MeSH
- Cell Proliferation drug effects MeSH
- Staphylococcus aureus drug effects growth & development MeSH
- Staphylococcus saprophyticus drug effects growth & development MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family. Phage vB_SsapS-104 showed a large latency period of about 40 min, rapid adsorption rate that was significantly enhanced by MgCl2 and CaCl2, and high stability to a wide range of temperatures and pH values. Restriction analyses demonstrated that phage consists of a double-stranded DNA with an approximate genome size of 40 Kb. BLAST results did not show high similarity (megablast) with other previously identified phages. But, in Blastn, similarity with Staphylococcus phages was observed. Phage vB_SsapS-104 represented high anti-bacterial activity against S. saprophyticus isolates in vitro as it was able to lyse 8 of the 9 clinical isolates (%88.8) obtained from a hospital in Gorgan, Iran. It was a S. saprophyticus-specific phage because no lytic activity was observed on some other pathogenic bacteria tested. Therefore, phage vB_SsapS-104 can be considered as a specific virulent phage against of S. saprophyitcus isolated from urinary tract infection. This study provided the partial genomic characterization of S. saprophyticus phage and its application against urinary tract infection associated with S. saprophyticus. This phage also can be considered as a good candidate for a therapeutic alternative in the future.
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- DNA, Viral MeSH
- Phage Therapy MeSH
- Genome, Viral MeSH
- Host Specificity MeSH
- Urinary Tract Infections microbiology MeSH
- Hydrogen-Ion Concentration MeSH
- Virus Latency MeSH
- Humans MeSH
- Wastewater virology MeSH
- Sequence Analysis, DNA MeSH
- Siphoviridae genetics isolation & purification ultrastructure MeSH
- Staphylococcus Phages genetics MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus saprophyticus drug effects virology MeSH
- Temperature MeSH
- Microscopy, Electron, Transmission MeSH
- Virulence MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Iran MeSH
- MeSH
- Bacterial Infections * diagnosis MeSH
- Bacteriological Techniques MeSH
- Candida albicans MeSH
- Adult MeSH
- Escherichia coli MeSH
- Laboratories MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Young Adult MeSH
- Specimen Handling MeSH
- Quality Control MeSH
- Shigella flexneri MeSH
- Staphylococcus aureus MeSH
- Staphylococcus saprophyticus MeSH
- Streptococcus pyogenes MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Male MeSH
- Female MeSH
- Publication type
- Case Reports MeSH
- Tables MeSH
