INTRODUCTION: Many widely used international histological textbooks claim that the epithelium of the human uterine tube consists of two, three, and, eventually, four types of cells. Most discrepancies among these textbooks relate to debates regarding the presence or absence of basal cells, whether the peg/intercalary cells and secretory cells are the same or distinct cell populations, and if the epithelium contains a population of immunologically active cells (T- and B-lymphocytes, NK cells, macrophages and dendritic cells) or dispersed endocrine cells. METHODS: Uterine tubes were obtained from 22 women (average age: 46.73 y) undergoing gynecological surgery. The women were in fertile age, mostly in the middle of the menstrual cycle (ovulation phase). Tissue samples were processed for immunohistochemistry using primary antibodies against proliferation markers (Ki67 and PCNA), immune system cells (CD1a, CD3, CD4, CD8, CD20, CD45RO, CD56, CD68, granzyme B and S100) and disperse endocrine cells (chromogranin A and synaptophysin). RESULTS: Most of the mature tubal epithelial cells, ciliated cells, and secretory cells were mitotically active (PCNA+), a population of basal undifferentiated cells was not identified. The dividing cells had a narrow-shaped nucleus (Ki67 positive). These cells were morphologically identical to - by the terminology mentioned - intercalary cells, assuming they represented actually dividing cells (epitheliocytus tubarius mitoticus). The tubal "basal cells" displayed small, hyperchromatic nuclei and very pale cytoplasm (clear cytoplasmic halo). They were located in the epithelium adjacent to the basement membrane, were non-mitotically active and their immunophenotype corresponded to intraepithelial regulatory T-lymphocytes (CD3+, CD8+, CD45RO+, CD4-, CD20-, CD56- and granzyme B-). Intraepithelial B-lymphocytes were only rarely identified. Intraepithelial NK cells, dendritic cells, macrophages and dispersed endocrine cells were not identified. CONCLUSIONS: We recommend replacing the term "epitheliocytus tubarius basalis" in the Terminologia Histologica with the term "lymphocytus T intraepithelialis tubarius", which represents intraepithelial regulatory T-cells (CD8+, CD45RO+) of the uterine tube. Additionally, we propose that intercalary/peg cells are actively dividing cells, instead of effete or degenerating cells. Finally, the histological nomenclature should be corrected in a way that peg/intercalary cells are not considered synonymous terms for secretory cells.
- MeSH
- antigen Ki-67 metabolismus MeSH
- CD antigeny analýza MeSH
- dospělí MeSH
- epitelové buňky klasifikace imunologie MeSH
- imunohistochemie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mitóza MeSH
- proliferace buněk MeSH
- proliferační antigen buněčného jádra metabolismus MeSH
- vejcovody anatomie a histologie cytologie imunologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Aim. This study evaluated the behavior of oral epithelial cells in contact with orthodontic appliances. Methods. Oral epithelial cells of clinically normal buccal mucosa were obtained by liquid-based exfoliative cytology from 22 orthodontic patients. The following regions were evaluated: a) oral mucosa exposed to friction from orthodontic brackets; b) oral mucosa exposed to friction from the tube on the orthodontic band, and c) oral mucosa not exposed to friction (control area). Nuclear (NA) and cytoplasmic (CA) areas and NA/CA ratio were assessed by an image analysis system. Cell morphology and cellularity were also analyzed by Papanicolaou technique. Results. The NA of the cells in contact with orthodontic brackets and bands were smaller than the control area. Cells in contact with the brackets showed the greatest reduction in CA in comparison with the cells subjected to friction from the tube, and the control group. Smears classified as type I predominated in all regions analyzed, although type II were predominant, together with superficial epithelial cells, mainly in the oral mucosa in contact with the band tube. Conclusion. Preclinical alterations in the epithelial cells of oral mucosa, caused by orthodontic appliances, are reduction in NA, increase in cell keratinization and inflammatory features, especially in the band tube area.
- MeSH
- bezobratlí MeSH
- biologické markery MeSH
- biomedicínský výzkum MeSH
- epidermální buňky MeSH
- epidermis MeSH
- epitelové buňky cytologie klasifikace metabolismus MeSH
- financování organizované MeSH
- galektin 3 diagnostické užití izolace a purifikace klasifikace MeSH
- galektiny * diagnostické užití izolace a purifikace klasifikace MeSH
- hlasové řasy cytologie MeSH
- Langerhansovy buňky cytologie MeSH
- lektiny diagnostické užití izolace a purifikace klasifikace MeSH
- lidé MeSH
- ligandy MeSH
- rohovka cytologie MeSH
- rostliny MeSH
- skvamocelulární nádory genetika imunologie MeSH
- statistika jako téma MeSH
- ústní sliznice cytologie MeSH
- Check Tag
- lidé MeSH
