The structure of the Fusarium culmorum mitogenome is similar to that of closely related Fusarium spp.: it has a total length of 103,844 bp, the base composition of the genome is the following: A (35.4%), T (32.9%), C (14.6%), and G (17.1%). The mitogenome contains 13 protein-coding genes, 2 ribosomal RNA (rRNA), and 28 transfer RNA (tRNA) genes, all coded on the same strand of DNA. The gene order is identical to that of the other Fusarium and Hypocreales mitogenomes. Maximum likelihood and Bayesian analysis based on the concatenated amino acid dataset of mitochondrial protein-coding genes confirmed close genetic relationship of F. culmorum to the other type B trichothecene producers F. graminearum and F. gerlachii.
Genetic diversity of the isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt collected from 12 states representing different agro-ecological regions of India was determined through randomly amplified polymorphic DNA (RAPD) markers. The UPGMA cluster analysis grouped the isolates into eight categories showing high magnitude of genetic diversity. Each group had the isolates from different states present in various agro-ecological regions of India. Therefore, the groups generated through the RAPD analysis were not corresponding to area of the origin of the isolates. The RAPD primers, namely, OPA 7 and OPA 11 produced Foc specific fragment of ≈1.3 kb and ≈1.4 kb, respectively in all the isolates. These fragments were eluted, purified, cloned in pGEM-T Easy vector and sequenced. Primers were designed with sequence information of these two fragments using primer.3 software. Two sets of sequence characterized amplified region markers (SC-FOC 1 and SC-FOC 2) developed from the sequences of these fragments were found to be specific to Foc and produced an amplicon of 1.3 and 1.4 kb, respectively. These set of markers were validated against the isolates of the pathogen collected from different locations of India representing various races of the pathogen. They are non-specific to the other Fusarium species, Rhizoctonia solani and R. bataticola.
- MeSH
- Cicer mikrobiologie MeSH
- DNA fungální chemie genetika MeSH
- DNA primery genetika MeSH
- Fusarium klasifikace genetika izolace a purifikace MeSH
- genetická variace MeSH
- genotyp MeSH
- molekulární typizace MeSH
- mykologické určovací techniky MeSH
- nemoci rostlin mikrobiologie MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- technika náhodné amplifikace polymorfní DNA MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indie MeSH
Genetic diversity of 11 representative isolates of Fusarium oxysporum f.sp. ciceris causing chickpea wilt was determined through internal transcribed spacer (ITS) region of the ribosomal DNA-restriction fragment length polymorphism (ITS-RFLP). ITS1+5.8s+ITS2 regions of the isolates were amplified with a set of primers ITS1 and ITS4 and amplified products were digested with 4 restriction enzymes (AluI, MboI, RsaI, MseI). Six different kinds of ITS-RFLP patterns were obtained. The ITS region of these isolates was sequenced and deposited to NCBI GeneBank. The nucleotide sequence homology of ITS region grouped the isolates into 5 categories. Primers were designed with sequence information using Primer 3 software. F. oxysporum f.sp. ciceris specific markers (FOC F2 and FOC R2) based on ITS region were developed for the first time for detection of the pathogen. The markers produced an amplicon of 292 bp; they were validated against the isolates of the pathogen collected from different locations of India.
- MeSH
- Cicer mikrobiologie MeSH
- DNA fingerprinting metody MeSH
- DNA fungální genetika chemie MeSH
- DNA primery genetika MeSH
- financování organizované MeSH
- Fusarium genetika izolace a purifikace klasifikace MeSH
- genetická variace MeSH
- mezerníky ribozomální DNA genetika chemie MeSH
- molekulární sekvence - údaje MeSH
- mykologie metody MeSH
- nemoci rostlin mikrobiologie MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- restrikční enzymy metabolismus MeSH
- sekvenční analýza DNA MeSH
- senzitivita a specificita MeSH
- Geografické názvy
- Indie MeSH
- MeSH
- dospělí MeSH
- fungemie etiologie MeSH
- Fusarium klasifikace MeSH
- infekce spojené se zdravotní péčí MeSH
- karcinom komplikace MeSH
- kontrola infekce MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory komplikace MeSH
- rizikové faktory MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
