The RP HPLC and LC/MS QTOF analyses of the methanolic CNS extract from isopod crustacean the woodlouse, Porcellio scaber revealed a presence of the red pigment-concentrating hormone (Panbo-RPCH) in this species. It has been shown that this neuropeptide plays a role in mobilization of energy stores: topical treatments of P. scaber individuals by Panbo-RPCH in a concentration 20 pmol/microl increased the level of glucose in haemolymph about 4 times, while the level of trehalose was only doubled. The results demonstrated that glucose was the main carbohydrate mobilized by the Panbo-RPCH treatment: glucose was responsible for about 97% of total carbohydrate increasing. Despite the demonstration of hyperglycaemic activity of Panbo-RPCH, no stimulatory effect of this hormone on the locomotory activity of P. scaber was observed. The present study is the first discovery of an occurrence of Panbo-RPCH and its hyperglycaemic activity in the representative of the isopod crustaceans. The relationship of the function of Panbo-RPCH in P. scaber to the role of this neuropeptide and adipokinetic hormones in insects is discussed.
- MeSH
- energetický metabolismus účinky léků fyziologie MeSH
- glukosa metabolismus MeSH
- hemolymfa metabolismus MeSH
- hormony bezobratlých MeSH
- Isopoda chemie fyziologie MeSH
- kyselina pyrrolidonkarboxylová aplikace a dávkování analogy a deriváty analýza MeSH
- oligopeptidy aplikace a dávkování analýza fyziologie MeSH
- pohybová aktivita účinky léků MeSH
- trehalosa metabolismus MeSH
- tukové těleso účinky léků metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
