High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay - ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied animals. DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain-fed animals, but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fed animals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days after the incorporation of corn into the diet and significantly lower concentration of bacteria belonging to Actinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day 20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantly higher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fed animals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals. The three different culture-independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet.
- MeSH
- Actinobacteria klasifikace genetika metabolismus MeSH
- bachor mikrobiologie MeSH
- Bacteroidetes klasifikace genetika metabolismus MeSH
- Butyrivibrio klasifikace genetika metabolismus MeSH
- Clostridium klasifikace genetika metabolismus MeSH
- fermentace MeSH
- Firmicutes klasifikace genetika metabolismus MeSH
- fylogeneze MeSH
- fyziologie výživy zvířat * MeSH
- koncentrace vodíkových iontů MeSH
- kozy MeSH
- krmivo pro zvířata analýza MeSH
- kukuřice setá chemie metabolismus MeSH
- lipnicovité chemie metabolismus MeSH
- píštěl žaludku MeSH
- Prevotella klasifikace genetika metabolismus MeSH
- Ruminococcus klasifikace genetika metabolismus MeSH
- sekvenční analýza DNA MeSH
- střevní mikroflóra fyziologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The expression of Ruminococcus flavefaciens 007S cellulases in different incubation time points (growth stages) and their substrate inducibility were analyzed by comparing the zymogram expression profiles of cultures grown on insoluble cellulose (Avicel) with cellobiose-grown cultures. The molecular weights of the enzymes were compared to (putative) cellulases encoded in the R. flavefaciens FD-1 genome.
- MeSH
- bakteriální proteiny chemie genetika metabolismus MeSH
- celobiosa metabolismus MeSH
- celulasy chemie genetika metabolismus MeSH
- celulosa metabolismus MeSH
- enzymatické testy MeSH
- exprese genu MeSH
- molekulární sekvence - údaje MeSH
- molekulová hmotnost MeSH
- Ruminococcus chemie enzymologie genetika růst a vývoj MeSH
- terciární struktura proteinů MeSH
- Publikační typ
- časopisecké články MeSH
