A panel of nine dibenzo[a,c]cyclooctadiene lignans, schizandrin, gomisin A, gomisin N, gomisin J, angeloylgomisin H, tigloylgomisin P, deoxyschizandrin, gamma-schizandrin and wuweizisu C was examined for their effect on multidrug resistance, as well as their anti-proliferative activities. COR-L23/R, a multidrug resistant sub-line, which has been reported to over-express multidrug resistance-associated protein (MRP1), was used for the experiments together with its parent cell line COR-L23 (human lung cell carcinoma). We found that lignans deoxyschizandrin and gamma-schizandrin at relatively non-toxic concentrations restored the cytotoxic action of doxorubicin to COR-L23/R cells. Deoxyschizandrin and gamma-schizandrin also significantly enhanced the accumulation of doxorubicin in drug resistant cells. Both lignans alone had no effect on the cell cycle; however, when combined with sub-toxic doses of doxorubicin, they induced cell cycle arrest in the G2/M phase, which is typical for toxic doses of doxorubicin. Our results suggest that deoxyschizandrin and gamma-schizandrin potentiate the cytotoxic effect of doxorubicin in doxorubicin resistant lung cancer cells COR-L23/R by increasing the accumulation of doxorubicin inside the cells. The common structural feature of both active lignans is the R-biaryl configuration and the absence of a hydroxy group at C-8. Unlike the reversal effect, the cytotoxicity of lignans with the R-biaryl configuration was similar to that observed for lignans with the S-biaryl configuration.

• MeSH
• antibiotika antitumorózní aplikace a dávkování   farmakologie   metabolismus   MeSH
• buněčné dělení účinky léků   MeSH
• chemorezistence účinky léků   MeSH
• cyklooktany aplikace a dávkování   farmakologie   chemie   MeSH
• daunomycin aplikace a dávkování   farmakologie   metabolismus   MeSH
• G2 fáze účinky léků   MeSH
• lidé MeSH
• lignany aplikace a dávkování   farmakologie   chemie   MeSH
• mnohočetná léková rezistence účinky léků   MeSH
• nádorové buněčné linie MeSH
• nádory plic farmakoterapie   metabolismus   MeSH
• velkobuněčný karcinom farmakoterapie   metabolismus   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• vztahy mezi strukturou a aktivitou MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• práce podpořená grantem MeSH