It is now more than nine years since Dolly, the world's first somatic cell cloned mammal was born, and the success of somatic cell nuclear transfer (SCNT) is still disappointingly low. Only about 3-5% of reconstructed embryos develop to term, and it is also evident that even if some clones are born, they are not necessarily fully developed and healthy. Embryonic and neonatal abnormalities of cloned offspring are probably a result of incorrect or incomplete reprogramming of the transferred donor cell nuclei. Such an incomplete reprogramming reflects the extremely low efficiency of SCNT. The key role in the process of reprogramming has been attributed to the enucleated oocyte-cytoplast into which the somatic cell nucleus is transferred. In our chapter, we will discuss the methodological approaches used for the preparation of cytoplasts and their possible reprogramming activities.
- MeSH
- buněčná diferenciace genetika MeSH
- buněčné jádro genetika MeSH
- cytoplazmatické struktury metabolismus MeSH
- embryonální vývoj genetika MeSH
- financování organizované MeSH
- histony genetika MeSH
- klonování organismů metody trendy MeSH
- lidé MeSH
- oocyty metabolismus MeSH
- restrukturace chromatinu genetika MeSH
- techniky jaderného přenosu normy trendy MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
