The aim of this work was to construct transgenic plants with increased capabilities to degrade organic pollutants, such as polychlorinated biphenyls. The environmentally important gene of bacterial dioxygenase, the bphC gene, was chosen to clone into a plant of Nicotiana tabacum. The chosen bphC gene encodes 2,3-dihydroxybiphenyl-1,2-dioxygenase, which cleaves the aromatic ring of dihydroxybiphenyl, and we cloned it in fusion with the gene for β-glucuronidase (GUS), luciferase (LUC) or with a histidine tail. Several genetic constructs were designed and prepared and the possible expression of desired proteins in tobacco plants was studied by transient expression. We used genetic constructs successfully expressing dioxygenase's genes we used for preparation of transgenic tobacco plants by agrobacterial infection. The presence of transgenic DNA , mRNA and protein was determined in parental and the first filial generation of transgenic plants with the bphC gene. Properties of prepared transgenic plants will be further studied.
- MeSH
- bakteriální proteiny genetika metabolismus MeSH
- biodegradace MeSH
- Burkholderiaceae enzymologie genetika MeSH
- dioxygenasy genetika metabolismus MeSH
- geneticky modifikované rostliny enzymologie genetika metabolismus MeSH
- glukuronidasa genetika metabolismus MeSH
- klonování DNA MeSH
- luciferasy genetika metabolismus MeSH
- polychlorované bifenyly metabolismus MeSH
- rekombinantní fúzní proteiny genetika metabolismus MeSH
- tabák enzymologie genetika metabolismus MeSH
- zelené fluorescenční proteiny genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
