A new microsporidian parasite Nosema chrysorrhoeae n. sp., isolated in Bulgaria from the browntail moth (Euproctis chrysorrhoea L.), is described. Its life cycle includes two sequential developmental cycles that are similar to the general developmental cycles of the Nosema-like microsporidia and are indistinguishable from those of two Nosema spp. from Lymantria dispar. The primary cycle takes place in the midgut tissues and produces binucleate primary spores. The secondary developmental cycle takes place exclusively in the silk glands and produces binucleate environmental spores. N. chrysorrhoeae is specific to the browntail moth. Phylogenetic analysis based on the ssu rRNA gene sequence places N. chrysorrhoeae in the Nosema/Vairimorpha clade, with the microsporidia from lymantriid and hymenopteran hosts. Partial sequences of the lsu rRNA gene and ITS of related species Nosema kovacevici (Purrini K., Weiser J., 1975. Natürliche Feinde des Goldafters, Euproctis chrysorrhoea L., im Gebiet von Kosovo, FSR Jugoslawien. Anzeiger fuer Schädlingskunde, Pflanzen-Umweltschutz, 48, 11-12), Nosema serbica Weiser, 1963 and Nosema sp. from Lymantria monacha was obtained and compared with N. chrysorrhoeae. The molecular data indicate the necessity of future taxonomic reevaluation of the genera Nosema and Vairimorpha.
- MeSH
- DNA fungální analýza genetika MeSH
- druhová specificita MeSH
- epitelové buňky mikrobiologie patologie ultrastruktura MeSH
- financování organizované MeSH
- fylogeneze MeSH
- geny rRNA genetika MeSH
- můry mikrobiologie MeSH
- Nosema genetika izolace a purifikace klasifikace růst a vývoj MeSH
- ribozomální DNA analýza genetika MeSH
- stadia vývoje MeSH
- svaly mikrobiologie patologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Bulharsko MeSH
When in vitro growth of Vittaforma corneae was tested using MDCK, MRC-5, XEN, L-929 and FHM cell lines, propagation occurred only in MDCK, MRC-5 and XEN cells. The intervals required for the various stages of the life cycle to develop were the same in all the cell lines tested. The MDCK cell line was selected to support the growth of V. corneae in vitro and provide the system for in vitro testing of drugs. The weekly output of V. corneae spores from the MDCK cell monolayer was monitored over a 61-week period during which there were fluctuations but no definite increase or decrease in output. Albendazole at 2.1 or 4.2 micrograms/ml in MEM was tested against V. corneae in MDCK cell monolayers and showed antimicrosporidial activity. The percentage of infected cells was reduced in the presence of the drug and there were ultrastructural abnormalities in all stages of the life cycle. The drug prevents parasite division.
- MeSH
- albendazol farmakologie MeSH
- anthelmintika farmakologie MeSH
- buněčné linie MeSH
- fibroblasty MeSH
- ledviny MeSH
- lidé MeSH
- Microsporida účinky léků růst a vývoj ultrastruktura MeSH
- Nosema účinky léků růst a vývoj ultrastruktura MeSH
- plíce embryologie MeSH
- psi MeSH
- spory MeSH
- Xenopus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
