The aim of this study was to determine possible differences in the faecal microbiota of dairy cows infected with Mycobacterium avium subsp. paratuberculosis (Johne's disease) in comparison with noninfected cows from the same herds. Faecal samples from cows in 4 herds were tested for M. avium subsp. paratuberculosis by real-time PCR, and faecal bacterial populations were analysed by 454 pyrosequencing of the 16S rRNA gene. The most notable differences between shedding and nonshedding cows were an increase in the genus Psychrobacter and a decrease in the genera Oscillospira, Ruminococcus, and Bifidobacterium in cows infected with M. avium subsp. paratuberculosis. The present study is the first to report the faecal microbial composition in dairy cows infected with M. avium subsp. paratuberculosis.

• MeSH
• feces mikrobiologie   MeSH
• mlékárenství MeSH
• Mycobacterium avium subsp. paratuberculosis genetika   izolace a purifikace   fyziologie   MeSH
• nemoci skotu mikrobiologie   MeSH
• paratuberkulóza mikrobiologie   MeSH
• sekvenční analýza DNA veterinární   MeSH
• skot mikrobiologie   MeSH
• vylučování bakterií z těla MeSH
• zvířata MeSH
• Check Tag
• skot mikrobiologie   MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH

The aims of this study were to describe spatial contamination of the environment on a mouflon pasture, as well as to assess the contamination of grass and roots after surface contamination and in depth contamination with feces and buried tissues from animals infected with Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis). Samples of soil, roots, and aerial parts of plants were collected from different locations inside the mouflon pasture, and one control sample site was chosen outside the area where the animals are living. M. a. paratuberculosis DNA was present in all the examined sites and was more often detected in roots than in soil. DNA was detected at up to 80 cm of depth and was spatially more widespread than the initial hypothesis of M. a. paratuberculosis leaching vertically into deeper layers of soil. This study broadens our knowledge of the spread and persistence of M. a. paratuberculosis in an environment with highly infected animals.

• MeSH
• krmivo pro zvířata analýza   mikrobiologie   MeSH
• lipnicovité mikrobiologie   MeSH
• Mycobacterium avium subsp. paratuberculosis genetika   izolace a purifikace   fyziologie   MeSH
• nemoci skotu mikrobiologie   přenos   MeSH
• paratuberkulóza mikrobiologie   přenos   MeSH
• půda chemie   MeSH
• půdní mikrobiologie * MeSH
• skot MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH