A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and Ixodes ricinus tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.

• MeSH
• buněčné linie MeSH
• fyziologická adaptace genetika   MeSH
• genetická variace MeSH
• genom virový MeSH
• klíště cytologie   virologie   MeSH
• klíšťová encefalitida virologie   MeSH
• ledviny cytologie   virologie   MeSH
• mutace MeSH
• myši MeSH
• prasata MeSH
• quasispecies * MeSH
• virulence MeSH
• viry klíšťové encefalitidy genetika   patogenita   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH