The plant-specific receptor-like cytoplasmic kinases (RLCKs) form a large, poorly characterized family. Members of the RLCK VI_A class of dicots have a unique characteristic: their activity is regulated by Rho-of-plants (ROP) GTPases. The biological function of one of these kinases was investigated using a T-DNA insertion mutant and RNA interference. Loss of RLCK VI_A2 function resulted in restricted cell expansion and seedling growth. Although these phenotypes could be rescued by exogenous gibberellin, the mutant did not exhibit lower levels of active gibberellins nor decreased gibberellin sensitivity. Transcriptome analysis confirmed that gibberellin is not the direct target of the kinase; its absence rather affected the metabolism and signalling of other hormones such as auxin. It is hypothesized that gibberellins and the RLCK VI_A2 kinase act in parallel to regulate cell expansion and plant growth. Gene expression studies also indicated that the kinase might have an overlapping role with the transcription factor circuit (PIF4-BZR1-ARF6) controlling skotomorphogenesis-related hypocotyl/cotyledon elongation. Furthermore, the transcriptomic changes revealed that the loss of RLCK VI_A2 function alters cellular processes that are associated with cell membranes, take place at the cell periphery or in the apoplast, and are related to cellular transport and/or cell wall reorganisation.

• MeSH
• Arabidopsis účinky léků   enzymologie   genetika   růst a vývoj   MeSH
• DNA bakterií genetika   metabolismus   MeSH
• DNA vazebné proteiny genetika   metabolismus   MeSH
• geneticky modifikované rostliny MeSH
• gibereliny metabolismus   farmakologie   MeSH
• hypokotyl účinky léků   enzymologie   genetika   růst a vývoj   MeSH
• inzerční mutageneze MeSH
• kotyledon účinky léků   enzymologie   genetika   růst a vývoj   MeSH
• kyseliny indoloctové metabolismus   farmakologie   MeSH
• protein-serin-threoninkinasy genetika   metabolismus   MeSH
• proteiny huseníčku genetika   metabolismus   MeSH
• regulace genové exprese u rostlin * MeSH
• regulátory růstu rostlin farmakologie   MeSH
• semenáček účinky léků   enzymologie   genetika   růst a vývoj   MeSH
• stanovení celkové genové exprese MeSH
• transkripční faktory bHLH genetika   metabolismus   MeSH
• transkripční faktory genetika   metabolismus   MeSH
• transkriptom MeSH
• vývojová regulace genové exprese MeSH
• Publikační typ
• časopisecké články MeSH