In this work, we compare labeling by two negatively charged fluorescent labels, 8-aminopyrene-1,3,6-trisulfonic acid (APTS) and 8-(2-hydrazino-2-oxoethoxy)pyrene-1,3,6-trisulfonic acid (Cascade Blue hydrazide [CBH]). Effectiveness of the labeling chemistries were investigated by 4-hydroxybenzaldehyde and maltoheptaose followed by LC/UV-MS and CE/LIF analysis, respectively. The reaction yield of APTS labeling was determined to be only ∼10%. This is due to reduction of almost 90% of the analyte by sodium cyanoborohydride to alcohol, which cannot be further labeled via reductive amination. However, the CBH labeling provides ∼90% reaction yield based on the LC/UV-MS measurements. The significantly higher labeling yield was also confirmed by CE/LIF measurements. Finally, the more effective hydrazone formation technique of CBH was characterized and applied for N-linked glycan analysis by CE/LIF.

• MeSH
• aminace MeSH
• chromatografie kapalinová metody   MeSH
• elektroforéza kapilární metody   MeSH
• fluorescenční barviva chemie   MeSH
• hmotnostní spektrometrie metody   MeSH
• hydrazony chemie   MeSH
• oligosacharidy analýza   chemie   MeSH
• pyreny chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH