Distances frequency distribution histogram
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3D super-resolution microscopy based on the direct stochastic optical reconstruction microscopy (dSTORM) with primary Alexa-Fluor-647-conjugated antibodies is a powerful method for accessing changes of objects that could be normally resolved only by electron microscopy. Despite the fact that mitochondrial cristae yet to become resolved, we have indicated changes in cristae width and/or morphology by dSTORM of ATP-synthase F1 subunit α (F1α). Obtained 3D images were analyzed with the help of Ripley's K-function modeling spatial patterns or transferring them into distance distribution function. Resulting histograms of distances frequency distribution provide most frequent distances (MFD) between the localized single antibody molecules. In fasting state of model pancreatic β-cells, INS-1E, MFD between F1α were ~80 nm at 0 and 3 mM glucose, whereas decreased to 61 nm and 57 nm upon glucose-stimulated insulin secretion (GSIS) at 11 mM and 20 mM glucose, respectively. Shorter F1α interdistances reflected cristae width decrease upon GSIS, since such repositioning of F1α correlated to average 20 nm and 15 nm cristae width at 0 and 3 mM glucose, and 9 nm or 8 nm after higher glucose simulating GSIS (11, 20 mM glucose, respectively). Also, submitochondrial entities such as nucleoids of mtDNA were resolved e.g. after bromo-deoxyuridine (BrDU) pretreatment using anti-BrDU dSTORM. MFD in distances distribution histograms reflected an average nucleoid diameter (<100 nm) and average distances between nucleoids (~1000 nm). Double channel PALM/dSTORM with Eos-lactamase-β plus anti-TFAM dSTORM confirmed the latter average inter-nucleoid distance. In conclusion, 3D single molecule (dSTORM) microscopy is a reasonable tool for studying mitochondrion.
- MeSH
- beta-buňky cytologie metabolismus MeSH
- buňky Hep G2 MeSH
- DNA vazebné proteiny metabolismus MeSH
- fluorescenční mikroskopie přístrojové vybavení MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- lidé MeSH
- mitochondriální DNA chemie metabolismus MeSH
- mitochondriální membrány metabolismus MeSH
- mitochondriální proteiny metabolismus MeSH
- potkani Wistar MeSH
- zobrazování trojrozměrné metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... Sealed Radioactive Sources and Orphan Sources Regulations 2005, 58 Protective Measures, 58 Time, 58 Distance ... ... Unit Formalism: Calculation of Monitor Units, 170 Treatment Plan Evaluation Tools, 170 Isodose Distributions ... ... , 170 Beams Eye View, 171 Dose Volume Histograms, 171 Other Tools, 173 -- Treatment Planning Techniques ... ... Control Required, 200 -- Commitment to Quality Control, 201 -- Safety, Position and Dose, 201 -- Frequency ...
Eighth edition xxiii, 615 stran : ilustrace, tabulky ; 28 cm
- MeSH
- nádory radioterapie MeSH
- nukleární lékařství metody MeSH
- radioterapie metody MeSH
- Publikační typ
- učebnice MeSH
- Konspekt
- Učební osnovy. Vyučovací předměty. Učebnice
- Lékařské vědy. Lékařství
- NLK Obory
- radiologie, nukleární medicína a zobrazovací metody
- onkologie
- NLK Publikační typ
- kolektivní monografie