We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.

• MeSH
• Agrobacterium tumefaciens enzymologie   genetika   metabolismus   MeSH
• alkany metabolismus   MeSH
• DNA bakterií chemie   genetika   MeSH
• estery metabolismus   MeSH
• hydrolasy genetika   izolace a purifikace   metabolismus   MeSH
• molekulární sekvence - údaje MeSH
• multimerizace proteinu MeSH
• rostliny mikrobiologie   MeSH
• sekvenční analýza DNA MeSH
• stereoizomerie MeSH
• substrátová specifita MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH