Phase separation forms membraneless compartments, including heterochromatin "domains" and repair foci. Pericentromeric heterochromatin mostly comprises repeated sequences prone to aberrant recombination. In Drosophila cells, "safe" homologous recombination (HR) repair of these sequences requires their relocalization to the nuclear periphery before Rad51 recruitment and strand invasion. How this mobilization initiates is unknown, and the contribution of phase separation is unclear. Here, we show that Nup98 nucleoporin is recruited to repair sites before relocalization by Sec13 or Nup88, and downstream of the Smc5/6 complex and heterochromatin protein 1 (HP1). Remarkably, Nup98 condensates are immiscible with HP1 condensates, and they are required and sufficient to mobilize repair sites and exclude Rad51, thus preventing aberrant recombination while promoting HR repair. Disrupting this pathway results in heterochromatin repair defects and widespread chromosome rearrangements, revealing an "off-pore" role for nucleoporins and phase separation in nuclear dynamics and genome integrity in a multicellular eukaryote.

• MeSH
• Chromosomal Proteins, Non-Histone metabolism   genetics   MeSH
• Drosophila melanogaster * genetics   metabolism   MeSH
• DNA Breaks, Double-Stranded MeSH
• Heterochromatin * genetics   metabolism   MeSH
• Chromobox Protein Homolog 5 MeSH
• Nuclear Pore Complex Proteins * metabolism   genetics   MeSH
• Cell Cycle Proteins metabolism   genetics   MeSH
• Drosophila Proteins * metabolism   genetics   MeSH
• Recombinational DNA Repair * MeSH
• Rad51 Recombinase * metabolism   genetics   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH