Radial growth, asexual sporulation, and cleistothecia formation as well as extracellular chitinase and proteinase formation of Aspergillus nidulans were monitored in surface cultures in order to study the physiological role of extracellular hydrolase production in carbon-stressed cultures. We set up carbon-stressed and carbon-overfed experimental conditions by varying the starting glucose concentration within the range of 2.5 and 40 g/L. Glucose starvation induced radial growth and hydrolase production and enhanced the maturation of cleistothecia; meanwhile, glucose-rich conditions enhanced mycelial biomass, conidia, and cleistothecia production. Double deletion of chiB and engA (encoding an extracellular endochitinase and a β-1,3-endoglucanase, respectively) decreased conidia production under carbon-stressed conditions, suggesting that these autolytic hydrolases can support conidia formation by releasing nutrients from the cell wall polysaccharides of dead hyphae. Double deletion of prtA and pepJ (both genes encode extracellular proteases) reduced the number of cleistothecia even under carbon-rich conditions except in the presence of casamino acids, which supports the view that sexual development and amino acid metabolism are tightly connected to each other in this fungus.

• MeSH
• Aspergillus nidulans enzymologie   růst a vývoj   MeSH
• celulasa genetika   MeSH
• chitinasy genetika   MeSH
• fungální proteiny genetika   MeSH
• glukosa chemie   MeSH
• hydrolasy genetika   MeSH
• hyfy růst a vývoj   MeSH
• kultivační média MeSH
• mutace MeSH
• proteasy genetika   MeSH
• regulace genové exprese enzymů MeSH
• regulace genové exprese u hub * MeSH
• spory hub růst a vývoj   MeSH
• vývojová regulace genové exprese MeSH
• Publikační typ
• časopisecké články MeSH