The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date.

• MeSH
• bakteriální proteiny chemie   MeSH
• barva MeSH
• ferrikyanidy chemie   MeSH
• konformace proteinů MeSH
• krystalografie rentgenová MeSH
• lakasa chemie   MeSH
• měď chemie   MeSH
• molekulární modely MeSH
• molekulární sekvence - údaje MeSH
• stabilita enzymů MeSH
• Streptomyces coelicolor enzymologie   MeSH
• vazebná místa MeSH
• železo chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH