The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human primary glioblastoma stem cells. Both methods allowed the isolation of all three types of nucleic acids and provided similar yields in mtDNA. However, the yield in ncDNA was more than tenfold higher on columns, as observed for both cell types. Conversely, the TRIzol method proved more reproducible and was the method of choice for isolating RNA from glioblastoma cells, as demonstrated for the housekeeping genes RPLP0 and RPS9.

• MeSH
• biochemie metody   MeSH
• buněčné jádro metabolismus   MeSH
• buňky Hep G2 MeSH
• glioblastom metabolismus   patologie   MeSH
• lidé MeSH
• messenger RNA izolace a purifikace   MeSH
• mitochondriální DNA izolace a purifikace   MeSH
• nádorové kmenové buňky metabolismus   MeSH
• reagenční diagnostické soupravy MeSH
• RNA izolace a purifikace   MeSH
• savci metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH