18062180 OR Piezoelectric immunosensor for the direct and rapid detection of Francisella tularensis Dotaz Zobrazit nápovědu
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detect F. tularensis with the limit of detection 10(5) CFU/mL with a typical measuring cycle > 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
- MeSH
- bioterorismus prevence a kontrola MeSH
- diagnostické techniky molekulární metody využití MeSH
- ELISA metody přístrojové vybavení využití MeSH
- finanční podpora výzkumu jako téma MeSH
- Francisella tularensis izolace a purifikace MeSH
- imunoanalýza metody přístrojové vybavení využití MeSH
- LD50 MeSH
- lidé MeSH
- lipopolysacharidy genetika chemická syntéza chemie MeSH
- polymerázová řetězová reakce metody přístrojové vybavení využití MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
