This study describes the prevalence of Encephalitozoon cuniculi in raw cow's milk and evaluates the effect of different milk pasteurization treatments on E. cuniculi infectivity for severe combined immunodeficient (SCID) mice. Using a nested polymerase chain reaction approach, 1 of 50 milking cows was found to repeatedly shed E. cuniculi in its feces and milk. Under experimental conditions, E. cuniculi spores in milk remained infective for SCID mice following pasteurization treatments at 72 °C for 15 s or 85 °C for 5 s. Based on these findings, pasteurized cow's milk should be considered a potential source of E. cuniculi infection in humans.

• MeSH
• Encephalitozoon cuniculi izolace a purifikace   patogenita   MeSH
• feces parazitologie   MeSH
• mléko parazitologie   MeSH
• myši SCID MeSH
• myši MeSH
• pasterizace metody   MeSH
• polymerázová řetězová reakce MeSH
• skot MeSH
• spory hub izolace a purifikace   patogenita   MeSH
• vysoká teplota MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH