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Encephalitozoon cuniculi in Raw Cow's Milk Remains Infectious After Pasteurization

M. Kváč, V. Tomanová, E. Samková, J. Koubová, M. Kotková, L. Hlásková, J. McEvoy, B. Sak,

. 2016 ; 13 (2) : 77-9. [pub] 20151209

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc17000793

This study describes the prevalence of Encephalitozoon cuniculi in raw cow's milk and evaluates the effect of different milk pasteurization treatments on E. cuniculi infectivity for severe combined immunodeficient (SCID) mice. Using a nested polymerase chain reaction approach, 1 of 50 milking cows was found to repeatedly shed E. cuniculi in its feces and milk. Under experimental conditions, E. cuniculi spores in milk remained infective for SCID mice following pasteurization treatments at 72 °C for 15 s or 85 °C for 5 s. Based on these findings, pasteurized cow's milk should be considered a potential source of E. cuniculi infection in humans.

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