Encephalitozoon cuniculi in Raw Cow's Milk Remains Infectious After Pasteurization
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26650923
DOI
10.1089/fpd.2015.2036
Knihovny.cz E-resources
- MeSH
- Encephalitozoon cuniculi isolation & purification pathogenicity MeSH
- Feces parasitology MeSH
- Milk parasitology MeSH
- Mice, SCID MeSH
- Mice MeSH
- Pasteurization methods MeSH
- Polymerase Chain Reaction MeSH
- Cattle MeSH
- Spores, Fungal isolation & purification pathogenicity MeSH
- Hot Temperature MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
This study describes the prevalence of Encephalitozoon cuniculi in raw cow's milk and evaluates the effect of different milk pasteurization treatments on E. cuniculi infectivity for severe combined immunodeficient (SCID) mice. Using a nested polymerase chain reaction approach, 1 of 50 milking cows was found to repeatedly shed E. cuniculi in its feces and milk. Under experimental conditions, E. cuniculi spores in milk remained infective for SCID mice following pasteurization treatments at 72 °C for 15 s or 85 °C for 5 s. Based on these findings, pasteurized cow's milk should be considered a potential source of E. cuniculi infection in humans.
References provided by Crossref.org
Chronic Infections in Mammals Due to Microsporidia
