Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting.

Centre of the Region Haná for Biotechnological and Agricultural Research Institute of Experimental Botany Šlechtitelů 31 783 71 Olomouc Czech Republic

Citace poskytuje Crossref.org

A linkage map of Aegilops biuncialis reveals significant genomic rearrangements compared to bread wheat

The genetic mechanism of B chromosome drive in rye illuminated by chromosome-scale assembly

A chromosome arm from Thinopyrum intermedium × Thinopyrum ponticum hybrid confers increased tillering and yield potential in wheat

Flow karyotyping of wheat-Aegilops additions facilitate dissecting the genomes of Ae. biuncialis and Ae. geniculata into individual chromosomes

Development of DNA Markers From Physically Mapped Loci in Aegilops comosa and Aegilops umbellulata Using Single-Gene FISH and Chromosome Sequences

Transcriptome reprogramming due to the introduction of a barley telosome into bread wheat affects more barley genes than wheat

Large-Scale Structural Variation Detection in Subterranean Clover Subtypes Using Optical Mapping

Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content

An advanced reference genome of Trifolium subterraneum L. reveals genes related to agronomic performance