- Publikační typ
- abstrakt z konference MeSH
Manipulačná liečba algických syndrómov krčnej chrbtice, vykonávaná nielen lekármi, ale aj inými zdravotníckymi pracovníkmi, je stále viac vyhľadávaná pacientmi ako alternatíva medikamentóznej liečby. I manipulačná liečba má však riziká. Jednou z najčastejšie popisovaných komplikácií v literatúre je vznik disekcie vertebrálnej artérie často končiacej jej oklúziou. Práca podáva literárny prehľad anatomických podmienok a možných mechanizmov vedúcich počas liečebnej manipulácie krčnej chrbtice k disekcii jednej z vertebrálnych artérií. Zároveň uvádza prehľad diagnostických možnosti s dôrazom na dostupnosť a špecifiká ultrasonografickej diagnostiky oklúzií vertebrálnych arterií. V závere sa práca venuje niektorým odporúčaniam zameraným na zníženie rizika možného vzniku poškodenia krčných artérií.
Manipulation therapy of aching syndromes of vertebral column performed by physicians or other medical personnel, is increasingly sought by patients as an alternative of medicament therapy. Even the manipulation treatment is not without risk. One of the most often reported complications in literature is the development of dissection of vertebral artery, frequently resulting in occlusion. The contribution reviews literature about anatomical conditions and possible mechanisms during therapeutic manipulation of cervical spine resulting in dissection of one of vertebral arteries. Moreover, the author presents the survey of diagnostic possibilities with emphasis to availability and specific features of ultrasound diagnostic od vertebral artery occlusion. In conclusion the contribution deals with some recommendations paying attention to a decrease of the risk of possible origin of arterial artery damage.
Calcium (Ca2+) signaling and the modulation of intracellular calcium ([Ca2+]i) levels play critical roles in several key processes that regulate cellular survival, growth, differentiation, metabolism, and death in normal cells. On the other hand, aberrant Ca2+-signaling and loss of [Ca2+]i homeostasis contributes to tumor initiation proliferation, angiogenesis, and other key processes that support tumor progression in several different cancers. Currently, chemically and functionally distinct drugs are used as chemotherapeutic agents in the treatment and management of cancer among which certain anti-cancer drugs reportedly suppress pro-survival signals and activate pro-apoptotic signaling through modulation of Ca2+-signaling-dependent mechanisms. Most importantly, the modulation of [Ca2+]i levels via the endoplasmic reticulum-mitochondrial axis and corresponding action of channels and pumps within the plasma membrane play an important role in the survival and death of cancer cells. The endoplasmic reticulum-mitochondrial axis is of prime importance when considering Ca2+-signaling-dependent anti-cancer drug targets. This review discusses how calcium signaling is targeted by anti-cancer drugs and highlights the role of calcium signaling in epigenetic modification and the Warburg effect in tumorigenesis.
- MeSH
- antitumorózní látky farmakologie terapeutické užití MeSH
- apoptóza účinky léků MeSH
- cílená molekulární terapie metody MeSH
- endoplazmatické retikulum účinky léků metabolismus MeSH
- lidé MeSH
- mitochondrie účinky léků metabolismus MeSH
- nádory farmakoterapie metabolismus MeSH
- proliferace buněk účinky léků MeSH
- vápník metabolismus MeSH
- vápníková signalizace účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
The receptor for the advanced glycation end products (RAGE) is a multiligand transmembrane receptor involved in chronic inflammation whose specific polymorphisms of the promoter gene were found to increase its transcriptional activity. We investigated the association of both allelic and genotypic -374T/A and -429T/C polymorphisms with inflammatory bowel disease. The STREGA guidelines were applied for planning and reporting. We enrolled 133 patients with Crohn's disease (CD), 149 with ulcerative colitis (UC), and 128 blood donors. Genomic DNA was extracted from peripheral blood leukocytes collected from each patient and control. RAGE polymorphisms were analyzed by PCR-restriction fragment length polymorphism assay. The Hardy-Weinberg equilibrium was first assessed, and then, the Kruskal-Wallis test and the Fisher exact test were used for etiologic group comparisons. Distribution of patients' characteristics across genotypes was evaluated by the Fisher exact test, while that across alleles was analyzed with a probit model. A 2-sided value of p < 0.05 was considered significant. Following the evidence of the Hardy-Weinberg equilibrium, we found a higher prevalence of the allele A of the -374T/A haplotype in UC (p = 0.043), and of the allele C of the -429T/C haplotype in CD (p < 0.001) with respect to the other groups. Moreover, the homozygous AA genotype of the -374T/A polymorphism resulted associated with late onset of CD, while its TT genotype with early onset (p = 0.049). The allele C of the 429T/C haplotype was associated with early onset of UC (p = 0.03), while a higher frequency of the heterozygous TC haplotype was found in those with pancolitis (p = 0.026). The differing distribution of these polymorphisms in healthy donors and CD/UC patients suggests a role in the development and outcome of these pathological conditions.
- MeSH
- dospělí MeSH
- frekvence genu MeSH
- genetická predispozice k nemoci * MeSH
- genotyp MeSH
- genotypizační techniky MeSH
- idiopatické střevní záněty genetika MeSH
- jednonukleotidový polymorfismus * MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- prevalence MeSH
- promotorové oblasti (genetika) * MeSH
- receptor pro konečné produkty pokročilé glykace genetika MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
PURPOSE: Phytochemicals are naturally occurring plant-derived compounds and some of them have the potential to serve as anticancer drugs. Based on recent evidence, aberrantly regulated expression of microRNAs (miRNAs) is closely associated with malignancy. MicroRNAs are characterized as small non-coding RNAs functioning as posttranscriptional regulators of gene expression. Accordingly, miRNAs regulate various target genes, some of which are involved in the process of carcinogenesis. RESULTS: This comprehensive review emphasizes the anticancer potential of phytochemicals, either isolated or in combination, mediated by miRNAs. The ability to modulate the expression of miRNAs demonstrates their importance as regulators of tumorigenesis. Phytochemicals as anticancer agents targeting miRNAs are widely studied in preclinical in vitro and in vivo research. Unfortunately, their anticancer efficacy in targeting miRNAs is less investigated in clinical research. CONCLUSIONS: Significant anticancer properties of phytochemicals as regulators of miRNA expression have been proven, but more studies investigating their clinical relevance are needed.
- MeSH
- antitumorózní látky fytogenní aplikace a dávkování farmakologie MeSH
- karcinogeneze účinky léků genetika MeSH
- lidé MeSH
- mikro RNA biosyntéza genetika MeSH
- nádory farmakoterapie genetika patologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
PURPOSE: To study the correlation between disorganization of inner retinal layer (DRIL) and macular thickness parameters, ellipsoid zone (EZ) disruption and retinal nerve fiber layer (RNFL) thickness on spectral domain optical coherence tomography (SD-OCT) in diabetic retinopathy (DR), for the first time. METHODS: A tertiary care center-based cross-sectional study was undertaken. One hundred and four consecutive study subjects of type 2 diabetes mellitus were included: diabetes mellitus with no retinopathy (No DR) (n = 26); non-proliferative DR (NPDR) (n = 26); proliferative DR (PDR) (n = 26) and healthy controls (n = 26). Best Corrected Visual Acuity (BCVA) was measured on the logarithm of the minimum angle of resolution (logMAR) scale. Clinician-friendly, SD-OCT based, grading systems were created for DRIL and EZ disruption, within the macular cube. DRIL was graded as: grade 0, DRIL absent; and grade 1, DRIL present. EZ disruption was graded as; Grade 0: Intact EZ; Grade 1: Focal disruption and Grade 2: Global disruption. Every study subject underwent RNFL thickness analysis. RESULTS: DRIL was significantly associated with increase in severity of DR.Pearson correlation analysis showed significant positive correlation between DRIL and CST CAT and grades of EZ disruption . However, a significant negative correlation was found between DRIL and RNFL thickness . CONCLUSION: Presence of DRIL correlates with severity of DR, EZ disruption and RNFL thinning.
- MeSH
- diabetes mellitus 2. typu komplikace MeSH
- diabetická retinopatie patologie patofyziologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- macula lutea patologie MeSH
- nervová vlákna fyziologie MeSH
- optická koherentní tomografie MeSH
- průřezové studie MeSH
- retina patologie patofyziologie MeSH
- zraková ostrost MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Background Several recent studies have reported the opportunity to diagnose significant narrowing of the coronary arteries without stress testing using local flow acceleration. Purpose To define how often patients with increased coronary flow velocities at rest (≥ 0.70 m/s) have a positive exercise echocardiography test. Material and Methods A total of 150 patients scheduled for exercise echocardiography were studied using transthoracic Doppler echocardiography in order to assess coronary artery flow velocity before exercise. Pulsed wave Doppler registered blood flow velocity placed on the color signal. The maximal diastolic velocity of coronary flow was measured. Results Of participants, 16% had a velocity of more than 0.70 m/s in the left main/proximal left anterior/proximal left circumflex arteries (LM/pLAD). A significant correlation was observed between the value of the maximal velocity in LM/pLAD and the ejection fraction at the peak of exercise ( r ≈ -0.39, P < 0.0001); between the value of the maximal velocity in LM/pLAD and index of wall motion abnormalities (IWMA) at the peak of exercise ( r ≈ 0.44, P < 0.0001); and between the value of the maximal velocity in LM/pLAD and dIWMA ( r ≈ 0.41, P < 0.0001). Afterwards, severe ischemia in stress echocardiography tests was observed in this group. The average IWMA of these tests was found to be 2.3. Sixty-two angiograms were available for comparison with Doppler data. Conclusion There is a significant correlation between the value of the maximal velocity in LM/pLAD/pLCx at rest and the severity of wall motion abnormalities during exercise tests.
- MeSH
- dopplerovská echokardiografie * MeSH
- hodnocení rizik MeSH
- koronární cirkulace * MeSH
- lidé středního věku MeSH
- lidé MeSH
- odpočinek MeSH
- prediktivní hodnota testů MeSH
- zátěžová echokardiografie * MeSH
- zátěžový test * MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Cieľom bolo zmapovať pomocou krokomerov pohybovú aktivitu zamestnancov so sedavým spôsobom práce a s telesnou aktivitou v zamestnaní. Počet krokov vo voľnom čase a v zamestnaní korelovať s údajmi o životospráve a pohybových návykoch. Metodika: Sledovaných bolo 186 probandov, sedavé zamestnanie malo 118 osôb (skupina A), zamestnanie s telesnou aktivitou 68 osôb (skupina B). Počet krokov zistený počas 4hodinového merania vo voľnom čase a v zamestnaní bol korelovaný s výsledkami niektorých antropometrických meraní a s odpoveïami v dotazníku zameranom na určenie povahy pohybovej činnosti v zamestnaní a vo voľnom čase. Výsledky: Vo vekovo porovnateľných súboroch sme zistili významný rozdiel (p < 0,003) v počte krokov vykonaných v zamestnaní medzi skupinou so sedavým zamestnaním (2 892 krokov) a zamestnaním s telesnou aktivitou (3 595 krokov). Rozdiel v počte krokov vo voľnom čase (A: 3 089, B: 2 402) nebol štatisticky významný (p = 0,151). V skupine B bol významný rozdiel (p < 0,0001) v počte krokov v zamestnaní (3 595) a vo voľnom čase (2 402), v skupineA bol rozdiel (v zamestnaní 2 892 krokov, vo voľnom čase 3 089 krokov) štatisticky menej významný (p < 0,003). V oboch skupinách (A aj B) bola priama súvislosť (p < 0,01) medzi BMI a % podielom tuku v tele v celom súbore, ako aj samostatne pre mužov a ženy. Pri rozdelení súboru podľa pohlavia bolo možné pozorovať u mužov aj u žien proporcionálny pokles počtu krokov (vo voľnom čase aj v zamestnaní) so stúpajúcim vekom. So stúpajúcim BMI klesal v oboch skupinách počet krokov vo voľnom čase aj v zamestnaní. Žiadna zo sledovaných skupín a podskupín nedosahovala literatúrou odporúčané počty krokov na deň.
The objective of this research was to measure the physical activity of employees with sedentary type of occupation (group A, 186 subjects) and employees with physical activity during work (group B, 68 subjects) by means of pedometers. Gained pedometric data were correlated with some anthropometric and enquiry data. A significant difference (p < 0,003) in the step counts during working time between the A group (2 892 steps) and B group (3 595 steps) was detected. The difference in step counts during leisure time (A: 3 089, B: 2 402) was insignificant (p = 0,151). In the group B, there was a highly significant difference (p < 0,0001) between the step count in working time (3 595) and in leisure time (2 402), whereas equal difference in the group A (2 892 / 3 089 steps) was less significant (p < 0,003). Both groups displayed direct association (p < 0,01) between BMI and the percentage body fat not only in the entire subject pool but also separately for men and women. If splitting the subjects according to their gender, both in men and in women a proportional decline of their step counts (in leisure time and in working time) was observed with increasing age. The step count in leisure time and in working time declined with increasing BMI in both genders.
- MeSH
- balneologie * metody organizace a řízení MeSH
- lidé MeSH
- muskuloskeletální systém * MeSH
- Check Tag
- lidé MeSH
