Anaplasma platys, the aetiological agent of infectious canine cyclic thrombocytopaenia, infects platelets of dogs, usually causing mild or asymptomatic disease. Although A. platys is transmitted by ticks, as for other Anaplasma species, alternative modes of transmission may be involved. The aim of the present study was to evaluate the occurrence of A. platys infection in litters of puppies, which could suggest possible vertical transmission. Twelve litters, together with the respective bitches, were included in the study for the detection of A. platys DNA by PCR, followed by sequencing. Five puppies, from 2/4 litters <28 days of age, tested positive for A. platys DNA. No puppies from eight litters 1-3 months of age tested positive for A. platys DNA. The identical sequences (16S rRNA and gltA partial gene), the absence of ticks on puppies at the time of collection and the young age of the five infected puppies suggest vertical transmission of A. platys. This mode of transmission might contribute to the maintenance and spread of the pathogen in canine populations.
- MeSH
- Anaplasma izolace a purifikace MeSH
- anaplasmóza diagnóza mikrobiologie přenos MeSH
- nemoci psů diagnóza mikrobiologie přenos MeSH
- psi MeSH
- sekvenční analýza DNA veterinární MeSH
- vertikální přenos infekce veterinární MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Anaplasma platys is a bacterium parasitic in the canine platelets, representing the causative agent of canine cyclic thrombocytopenia, with a worldwide distribution, mainly in tropical countries. The agent has zoonotic potential, being reported in several human clinical cases. The suspected vector is the tick Rhipicephalus sanguineus (sensu lato), widely distributed in animals and humans in the tropical regions of South America, Africa, Asia and Australia, but also in southern Europe. Only few scattered data concerning the epidemiology of A. platys in sub-Saharan Africa are available. The aim of our study was to evaluate the epidemiological status of A. platys in dogs and cats from rural communities in eastern and western Africa, where dogs and their ticks live in close contact with humans. METHODS: A total of 216 canine and 22 feline blood samples and ticks feeding on them were collected in 2013 and 2014 from eight localities in Ivory Coast and four localities in Kenya. PCR was performed using specific primers targeting a portion of the 16S rRNA gene, followed by sequencing. RESULTS: The main results highlight the high prevalence of A. platys infection in dogs in both Eastern and Western Africa and report it for the first time in Eastern Africa and in Rhipicephalus camicasi. CONCLUSIONS: The presence of A. platys DNA in R. camicasi feeding on negative hosts together with the relatively high prevalence of A. platys, correlated with the absence of the probable vector Rhipicephalus sanguineus (s.l.) in Kenyan Island suggest the involvement of other tick species in the transmission of A. platys.
- MeSH
- Anaplasma izolace a purifikace MeSH
- anaplasmóza mikrobiologie MeSH
- DNA bakterií chemie genetika MeSH
- infestace klíšťaty veterinární MeSH
- kočky MeSH
- krev mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- psi MeSH
- Rhipicephalus růst a vývoj mikrobiologie MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- venkovské obyvatelstvo MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Keňa MeSH
- Pobřeží slonoviny MeSH
Anaplasma phagocytophilum is a worldwide distributed bacterium with a significant medical and veterinary importance. It grows within the phagosome of infected neutrophils and is responsible for human granulocytic anaplasmosis (HGA), tick-borne fever (TBF) of small ruminants and cattle, canine and equine granulocytic anaplasmosis, but infects also a great variety of wildlife species. Wild ungulates and rodents are considered reservoirs of infection in natural foci. The objective of this study was to determine the spectrum of animal species involved in the circulation of A. phagocytophilum in Slovakia and to analyze the variability of obtained nucleotide sequences, in order to determine whether genotypes from Slovakia cluster according to host-species or geographical location. Several animal species and vector ticks were screened for the presence of members of the family Anaplasmataceae using PCR based methods. Additional data on the molecular evidence of Anaplasma ovis and Candidatus Neoehrlichia mikurensis are presented. These pathogens were detected in tested sheep flocks and rodents with the mean infection rates of 8.16% and 10.75%, respectively. A. phagocytophilum was genotyped by 16S rRNA and groEL gene sequencing. Bacterial DNA was confirmed in questing ixodid ticks, in domesticated canine, wild rodents and several species of wild ungulates. In European isolates, 16S rRNA gene does not seem to be an appropriate locus for the analyses of heterogeneity as it is too conservative. Similarly, 16S rRNA isolates from our study did not reveal any polymorphisms. All isolates were identical in overlapped region and showed identity with sequences from ticks, horses or ruminants previously isolated elsewhere in the world. On the other hand, the groESL heat shock operon is widely used for determination of diversity and the analyses have already revealed considerable degree of heterogeneity. Tested ungulates were infected with A. phagocytophilum to a considerable extent. High proportions of red and roe deer tested positive and the rates of infection reached over 60.0%. GroEL sequences from canine, wild ungulates and ticks from Slovakia clustered within a clade together with isolates from horses, humans, wild ungulates and ticks from Slovakia or elsewhere in the world. Sequences from rodents clustered apart from those obtained from wild ungulates, ticks and humans. These results suggest that European rodents do not harbour A. phagocytophilum strains with strong zoonotic potential such as those from United States.
- MeSH
- Anaplasma klasifikace genetika izolace a purifikace MeSH
- anaplasmóza MeSH
- arachnida jako vektory mikrobiologie MeSH
- hlodavci MeSH
- klíště mikrobiologie MeSH
- psi MeSH
- vysoká zvěř MeSH
- zdroje nemoci mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika MeSH
Distribution of Anaplasma spp., Babesia spp., Theileria spp., and Ehrlichia ruminantium, was for the first time studied in Bié Province, central Angola. We examined 76 blood samples of cattle originated from seven farms, and 13 blood samples of goats from two farms employing molecular genetic tools (PCR). Most prevalent was A. ovis-infection in goats (100%) and A. marginale-infection in cattle (38% of examined animals, and six out of seven farms). B. bigemina-infection was detected in only one specimen at Andulo, whereas B. bovis was not detected in Bié. We did not detected T. parva, the causative agent of serious diseases in cattle; nevertheless, infection by T. velifera was quite frequent (14% of examined animals, and five out of seven farms). Causative agent of heartwater disease - E. ruminantium, was not detected. Taking into account short-term perspective of PCR methods in monitoring of epidemiological status in herds, the number of infected animals and distribution of detected pathogens should not be ignored.
- MeSH
- Anaplasma genetika izolace a purifikace MeSH
- anaplasmóza epidemiologie mikrobiologie MeSH
- Babesia genetika izolace a purifikace MeSH
- babezióza epidemiologie parazitologie veterinární MeSH
- DNA bakterií krev chemie izolace a purifikace MeSH
- kozy MeSH
- nemoci koz epidemiologie mikrobiologie parazitologie MeSH
- nemoci přenášené klíšťaty epidemiologie mikrobiologie parazitologie veterinární MeSH
- nemoci skotu epidemiologie mikrobiologie parazitologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- protozoální DNA krev chemie izolace a purifikace MeSH
- sekvence nukleotidů MeSH
- skot MeSH
- Theileria genetika izolace a purifikace MeSH
- theilerióza epidemiologie parazitologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Angola MeSH
- MeSH
- Anaplasma genetika izolace a purifikace MeSH
- Bacteria izolace a purifikace MeSH
- Borrelia genetika izolace a purifikace MeSH
- Coxiella burnetii genetika izolace a purifikace MeSH
- finanční podpora výzkumu jako téma MeSH
- klíšťata mikrobiologie MeSH
- nemoci přenášené klíšťaty mikrobiologie MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- Anaplasma izolace a purifikace MeSH
- Borrelia burgdorferi komplex izolace a purifikace MeSH
- DNA bakterií MeSH
- ehrlichióza epidemiologie mikrobiologie MeSH
- finanční podpora výzkumu jako téma MeSH
- lidé MeSH
- lymeská nemoc epidemiologie MeSH
- zdroje nemoci MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Geografické názvy
- Slovenská republika MeSH
