The aim of the present study was to assess the prevalence of Coxiella burnetii in dairy herds in Central and Eastern European countries based on ELISA and PCR tests. A total of 370 bulk tank milk samples were collected in 2019 originating from Croatia (n = 13), Czech Republic (n = 138), Hungary (n = 126), Serbia (n = 24), Slovakia (n = 53) and Slovenia (n = 16). Prevalence of C. burnetii differed according to the country of origin with Croatia showing 100.00%, the Czech Republic 98.55%, Hungary 97.61%, Serbia 70.83%, Slovakia 90.56% and Slovenia showing 62.50% average percentages of the positive herds. C. burnetii specific ELISA showed 100.00% positivity in all examined countries if herds consisted of equal or above 250 milking cows. The growing number of farms managing large number of animals, where cattle density is high correlates with the increasing prevalence of C. burnetii in the region.
- MeSH
- Coxiella burnetii * genetika MeSH
- mlékárenství MeSH
- nemoci skotu * epidemiologie MeSH
- prevalence MeSH
- Q-horečka * epidemiologie veterinární MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Chorvatsko MeSH
- Srbsko MeSH
Q fever is a zoonosis. Humans are infected through the inhalation of Coxiella burnetii particles that are dispersed into the air from the birth products or faeces of ruminants. Major outbreaks can occur in association with farming activities. C. burnetii can be disseminated by wind up to several tens of kilometres and infect humans far from its zoonotic source. As a result, the sources of sporadic cases are rarely identified. We report a sporadic case of acute Q fever in a French farmer returning from a cruise in the Caribbean. Careful examination found that the infection was not associated with travel, and a veterinary investigation identified C. burnetii DNA (MST genotype 8) in the faeces, nasal and vaginal swabs of several ewes from her herd of sheep. As a consequence, the herd was slaughtered to avoid dissemination of the infection.
- MeSH
- Coxiella burnetii genetika izolace a purifikace MeSH
- genotyp MeSH
- hospodářská zvířata MeSH
- lidé středního věku MeSH
- lidé MeSH
- nemoci ovcí diagnóza prevence a kontrola přenos MeSH
- ovce MeSH
- Q-horečka diagnóza prevence a kontrola přenos MeSH
- zoonózy diagnóza prevence a kontrola přenos MeSH
- zvířata MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
Coxiella burnetii (C. burnetii), an intracellular zoonotic bacterium causing Q fever, occurs widely in cattle herds. After invasion of the pregnant uterus and initial localization in the placenta, active C. burnetii infections may spread to the fetus hematogenously or by the amniotic-oral route and thus may cause abortion, premature delivery, stillbirth, and weak offspring (APSW) complex. In a case-control study, we investigated precolostral blood samples of 56 stillborn calves and 30 live births from a dairy herd endemically infected with C. burnetii "C-cluster" strains and an increased stillbirth rate in primiparous cows. Within the group of the stillborn calves, four precolostral blood samples (7.1%) were tested positive for C. burnetii DNA by PCR and one serum sample (1.8%) positive for anti-C. burnetii IgG antibodies by a commercial ELISA test, respectively. Neither C. burnetii DNA nor anti-C. burnetii IgG antibodies were detected in the samples of calves being born alive. In conclusion, we demonstrated that coxiellaemia and precolostral seroconversion occurred sporadically in stillborn calves from this endemically infected herd. Due to the low detection rates, C. burnetii could not be confirmed to be the cause of the increased stillbirth rate.
- MeSH
- Coxiella burnetii genetika imunologie izolace a purifikace MeSH
- DNA bakterií genetika MeSH
- imunoglobulin G krev MeSH
- narození mrtvého plodu veterinární MeSH
- nemoci skotu krev diagnóza embryologie mikrobiologie MeSH
- protilátky bakteriální krev MeSH
- Q-horečka diagnóza embryologie mikrobiologie veterinární MeSH
- skot MeSH
- studie případů a kontrol MeSH
- těhotenství MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- Anaplasma genetika izolace a purifikace MeSH
- Bacteria izolace a purifikace MeSH
- Borrelia genetika izolace a purifikace MeSH
- Coxiella burnetii genetika izolace a purifikace MeSH
- finanční podpora výzkumu jako téma MeSH
- klíšťata mikrobiologie MeSH
- nemoci přenášené klíšťaty mikrobiologie MeSH
- sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů metody MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- Coxiella burnetii genetika izolace a purifikace patogenita MeSH
- klíšťata mikrobiologie MeSH
- kozy mikrobiologie MeSH
- lidé MeSH
- plazmidy MeSH
- Q-horečka mikrobiologie MeSH
- restrikční mapování MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
