- MeSH
- antibakteriální látky terapeutické užití MeSH
- diagnostické techniky a postupy klasifikace MeSH
- hnisání klasifikace patologie MeSH
- infekce spojené se zdravotní péčí etiologie klasifikace prevence a kontrola MeSH
- infekce * klasifikace patofyziologie terapie MeSH
- infekční nemoci kůže klasifikace patologie terapie MeSH
- lidé MeSH
- nitrobřišní infekce klasifikace patologie terapie MeSH
- ruka patologie MeSH
- zánět patofyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
- Klíčová slova
- konzervatívna liečba, chirurgická liečba,
- MeSH
- dítě MeSH
- hnisání komplikace ošetřování patologie prevence a kontrola MeSH
- lidé MeSH
- malé chirurgické výkony metody MeSH
- mladiství MeSH
- palec nohy abnormality růst a vývoj MeSH
- zarostlé nehty * diagnóza etiologie chirurgie ošetřování MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
Impaired diabetic wound healing is an important current medical issue, mainly concerning patients recovering from complicated operations or patients with ulcers on their feet. The obese Zucker diabetic fatty rat, with a mutation in leptin receptors, may be a good choice for studying impaired wound healing. Male and female rats were fed a diabetogenic high-fat diet. Wound size changes of air-exposed excisional 2 cm circular wounds were measured until Day 10. Wound tissue was analyzed morphologically, histologically, and immunohistochemically. The hydroxyproline content in the granulation tissue (GT) was determined. mRNA expression was assayed by DNA-array analysis and real-time reverse transcription-polymerase chain reaction. Wound-size changes were retarded in diabetic rats and differed between the sexes. Diabetic wounds were characterized by impaired contraction, abundant crust production, increased inflammation, and pus formation. On Day 10, the GT contained a significantly increased amount of intercalated fat tissue and showed an irregular arrangement of GT and collagen fibers. Interestingly, the length of new epithelium was increased in diabetic wounds. The concentration of hydroxyproline in the GT of diabetic animals was significantly decreased to about one half when compared with the nondiabetic controls. The expression of interleukin-6, myeloperoxidase, stromelysin-1, and collagenase-3 was increased in the GT of diabetic rats on Day 10, while the expression of type I collagen and elastin was decreased. Taken together, Zucker diabetic fatty rats exhibited impairments in wound-size reduction, inflammatory response, tissue organization, and connective tissue turnover and are thus proposed as a new model for studying impaired repair.
- MeSH
- diabetes mellitus 2. typu patofyziologie MeSH
- elastin metabolismus MeSH
- granulační tkáň metabolismus patologie MeSH
- hnisání patologie MeSH
- hojení ran fyziologie MeSH
- hydroxyprolin metabolismus MeSH
- interleukin-6 metabolismus MeSH
- kolagen typu I metabolismus MeSH
- krysa rodu rattus MeSH
- kůže zranění patologie MeSH
- matrixová metaloproteinasa 13 metabolismus MeSH
- matrixová metaloproteinasa 3 metabolismus MeSH
- modely nemocí na zvířatech MeSH
- obezita patofyziologie MeSH
- peroxidasa metabolismus MeSH
- potkani Zucker MeSH
- sexuální faktory MeSH
- zánět patologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
