In an increasingly aging society, there is a growing demand for the development of technology related to tissue regeneration. It involves the development of the appropriate biomaterials whose properties will allow the desired biological response to be obtained. Bioactivity is strongly affected by the proper selection of active ingredients. The aim of this study was to produce bioactive hydrogel materials based on hyaluronic acid and collagen modified by the addition of placenta. These materials were intended for use as dressings, and their physicochemical properties were investigated under simulated biological environmental conditions. The materials were incubated in vitro in different fluids simulating the environment of the human body (e.g., simulated body fluid) and then stored at a temperature close to body temperature. Using an FT-IR spectrophotometer, the functional groups present in the composites were identified. The materials with the added placenta showed an increase in the swelling factor of more than 300%. The results obtained confirmed the potential of using this material as an absorbent dressing. This was indicated by pH and conductometric measurements, sorption, degradation, and surface analysis under an optical microscope. The results of the in vitro biological evaluation confirmed the cytosafety of the tested biomaterials. The tested composites activate monocytes, which may indicate their beneficial properties in the first phases of wound healing. The material proved to be nontoxic and has potential for medical use.
- MeSH
- biokompatibilní materiály farmakologie MeSH
- hojení ran MeSH
- hydrogely * farmakologie chemie MeSH
- kolagen farmakologie chemie MeSH
- kyselina hyaluronová * farmakologie chemie MeSH
- lidé MeSH
- ovce MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Hybrid biocomposite scaffolds (HBS) that serve as a carrier for cell proliferation and differentiation are increasingly used for tissue regeneration. 3D hybrid scaffold based on collagen-grafted-chitosan-glucan fiber (CO-g-CGF-HBS) was prepared by freeze-drying technique. The swelling percentage, hydrolytic stability, and modulus of elasticity of HBS were enhanced after the chemical modification of CO with CGF. Pore size and porosity of HBS were decreased with an increased CGF ratio. HBS exhibits a higher reduction rate against different types of bacteria compared with a control sample. Thus, chemical modification of CO with different ratios of CGF significantly improved the physicochemical, antibacterial properties of HBS.
Congenital clubfoot is a complex musculoskeletal deformity, in which a stiff, contracted tissue forms in the medial part of the foot. Fibrotic changes are associated with increased collagen deposition and lysyl oxidase (LOX)-mediated crosslinking, which impair collagen degradation and increase the tissue stiffness. First, we studied collagen deposition, as well as the expression of collagen and the amount of pyridinoline and deoxypyridinoline crosslinks in the tissue of relapsed clubfoot by immunohistochemistry, real-time PCR, and enzyme-linked immunosorbent assay (ELISA). We then isolated fibroblast-like cells from the contracted tissue to study the potential inhibition of these processes in vitro. We assessed the effects of a LOX inhibitor, β-aminopropionitrile (BAPN), on the cells by a hydroxyproline assay, ELISA, and Second Harmonic Generation imaging. We also evaluated the cell-mediated contraction of extracellular matrix in 3D cell-populated collagen gels. For the first time, we have confirmed significantly increased crosslinking and excessive collagen type I deposition in the clubfoot-contracted tissue. We successfully reduced these processes in vitro in a dose-dependent manner with 10-40 µg/mL of BAPN, and we observed an increasing trend in the inhibition of the cell-mediated contraction of collagen gels. The in vitro inhibitory effects indicate that BAPN has good potential for the treatment of relapsed and resistant clubfeet.
- MeSH
- aminopropionitril farmakologie MeSH
- fibroblasty účinky léků MeSH
- kolagen chemie MeSH
- lidé MeSH
- lysyloxidasa antagonisté a inhibitory MeSH
- pes equinovarus farmakoterapie metabolismus patologie MeSH
- předškolní dítě MeSH
- reagencia zkříženě vázaná farmakologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity of the graft is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling of the scaffold by the patient's cells should result in the formation of living autologous tissue. In the work presented here, decellularized porcine pericardium scaffolds were modified by growing a fibrin mesh on the surface and inside the scaffolds, and by attaching heparin and human vascular endothelial growth factor (VEGF) to this mesh. Then the scaffolds were seeded with human adipose tissue-derived stem cells (ASCs). While the ASCs grew only on the surface of the decellularized pericardium, the fibrin-modified scaffolds were entirely repopulated in 28 d, and the scaffolds modified with fibrin, heparin and VEGF were already repopulated within 6 d. Label free mass spectrometry revealed fibronectin, collagens, and other extracellular matrix proteins produced by ASCs during recellularization. Thin layers of human umbilical endothelial cells were formed within 4 d after the cells were seeded on the surfaces of the scaffold, which had previously been seeded with ASCs. The results indicate that an artificial tissue prepared by in vitro recellularization and remodeling of decellularized non-autologous pericardium with autologous ASCs seems to be a promising candidate for cardiovascular grafts capable of accelerating in situ endothelialization. ASCs resemble the valve interstitial cells present in heart valves. An advantage of this approach is that ASCs can easily be collected from the patient by liposuction.
- MeSH
- bioprotézy MeSH
- decelularizovaná extracelulární matrix chemie MeSH
- endoteliální buňky pupečníkové žíly (lidské) MeSH
- endoteliální buňky cytologie MeSH
- extracelulární matrix metabolismus MeSH
- fibrinogen chemie MeSH
- fibronektiny chemie MeSH
- fluorescenční mikroskopie MeSH
- kmenové buňky MeSH
- kolagen chemie MeSH
- lidé MeSH
- lipektomie MeSH
- perikard metabolismus patologie MeSH
- prasata MeSH
- proliferace buněk MeSH
- srdeční chlopně * MeSH
- techniky in vitro MeSH
- thrombin chemie MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury * chemie MeSH
- tuková tkáň cytologie MeSH
- vaskulární endoteliální růstový faktor A metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Archaeological research is radically transforming the view that the Amazon basin and surrounding areas witnessed limited societal development before European contact. Nevertheless, uncertainty remains on the nature of the subsistence systems and the role that aquatic resources, terrestrial mammalian game, and plants had in supporting population growth, geographic dispersal, cultural adaptations and political complexity during the later stages of the pre-Columbian era. This is exacerbated by the general paucity of archaeological human remains enabling individual dietary reconstructions. Here we use stable carbon and nitrogen isotope analysis of bone collagen to reconstruct the diets of human individuals from São Luís Island (Brazilian Amazon coast) dated between ca. 1800 and 1000 cal BP and associated with distinct ceramic traditions. We expanded our analysis to include previously published data from Maracá and Marajó Island, in the eastern Amazon. Quantitative estimates of the caloric contributions from food groups and their relative nutrients using a Bayesian Mixing Model revealed distinct subsistence strategies, consisting predominantly of plants and terrestrial mammals and variably complemented with aquatic resources. This study offers novel quantitative information on the extent distinct food categories of polyculture agroforestry systems fulfilled the caloric and protein requirements of Late Holocene pre-Columbian populations in the Amazon basin.
- MeSH
- archeologie metody MeSH
- Bayesova věta MeSH
- dějiny 15. století MeSH
- dějiny 16. století MeSH
- dějiny 17. století MeSH
- dějiny starověku MeSH
- dějiny středověku MeSH
- dieta dějiny MeSH
- izotopy dusíku analýza MeSH
- izotopy uhlíku analýza MeSH
- kolagen chemie MeSH
- kosti a kostní tkáň chemie MeSH
- lidé MeSH
- přijímání potravy MeSH
- rostliny MeSH
- savci MeSH
- stravovací zvyklosti MeSH
- tělesné pozůstatky MeSH
- zvířata MeSH
- Check Tag
- dějiny 15. století MeSH
- dějiny 16. století MeSH
- dějiny 17. století MeSH
- dějiny starověku MeSH
- dějiny středověku MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- historické články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Brazílie MeSH
OBJECTIVES: This study aimed to reconstruct the dietary behavior of two early medieval individuals who display gnathic malformation. MATERIAL: Two skeletons affected by temporomandibular ankylosis were analyzed, one from the Great Moravian burial site of Rajhradice (9th century AD, Czech Republic), and the other from the Avar burial site of Schӧnkirchen (8th century AD, Austria). METHODS: Carbon and nitrogen isotopic values were measured from the bone collagen of both individuals. In the Rajhradice case, where the childhood origin of ankylosis is deduced, isotopic analysis of dentine sections was performed. RESULTS: Both individuals show isotopic values within the range of variation of a contemporaneous population sample. There was no observable dietary change in the Rajhradice individual that could be linked to the occurrence of ankylosis. CONCLUSIONS: Both individuals consumed diets typical for their populations. They appear to not have restricted access to foodstuffs, namely animal protein, which would likely have had to be served in liquid (e.g. milk) or in a highly mashed form to compensate for insufficient mastication. SIGNIFICANCE: This finding provides specific evidence of care provided to these two afflicted members of past populations. LIMITATIONS: Though the proportion of animal protein is an important indicator of the quality of diet, many other aspects of diet - such as micronutrient content - elude stable isotope analysis. SUGGESTIONS FOR FURTHER RESEARCH: Amino acid compound specific isotope analyses of collagen would provide deeper insight into both the diet and physiology of the affected individuals.
- MeSH
- ankylóza * etnologie patologie MeSH
- dějiny středověku MeSH
- dieta etnologie MeSH
- dospělí MeSH
- kolagen chemie MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- nemoci temporomandibulárního kloubu * etnologie patologie MeSH
- paleopatologie MeSH
- temporomandibulární kloub patologie MeSH
- Check Tag
- dějiny středověku MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- historické články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Rakousko MeSH
Every year, the poultry industry produces a large number of by-products such as chicken heads containing a considerable proportion of proteins, particularly collagen. To prepare gelatin is one of the possibilities to advantageously utilize these by-products as raw materials. The aim of the paper was to process chicken heads into gelatins. An innovative method for conditioning starting raw material was using the proteolytic enzyme. Three technological factors influencing the yield and properties of extracted gelatins were monitored including the amount of enzyme used in the conditioning of the raw material (0.4% and 1.6%), the time of the conditioning (18 and 48 h), and the first gelatin extraction time (1 and 4 h). The gelatin yield was between 20% and 36%. The gelatin gel strength ranged from 113 to 355 Bloom. The viscosity of the gelatin solution was determined between 1.4 and 9.5 mPa.s. The content of inorganic solids varied from 2.3% to 3.9% and the melting point of the gelatin gel was recorded between 34.5 and 42.2 °C. This study has shown that gelatin obtained from chicken heads has a promising potential with diverse possible applications in the food industry, pharmacy, and cosmetics.
- MeSH
- časové faktory MeSH
- gely chemie izolace a purifikace MeSH
- hlava MeSH
- kolagen chemie metabolismus MeSH
- kur domácí metabolismus MeSH
- potraviny MeSH
- proteasy MeSH
- průmysl zpracování potravin přístrojové vybavení metody MeSH
- viskozita MeSH
- želatina analýza chemie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
For the pretreatment of wood, charcoal and collagen from bone micro samples using the Acid-Base-Acid (ABA) method, we have assembled an automated computer-controlled unit in our laboratory CRL. The sample is placed in a glass single-necked cuvette. The machine consists of prepared solutions which are guided through capillaries, switching valve and peristaltic pump into the cuvette with the sample according to the currently selected program. The automat can be used for the pretreatment of charcoal, wood and also collagen from bones.
- MeSH
- alkálie chemie MeSH
- dřevěné a živočišné uhlí chemie MeSH
- dřevo chemie MeSH
- kolagen chemie MeSH
- kosti a kostní tkáň chemie MeSH
- kyseliny chemie MeSH
- laboratorní automatizace metody MeSH
- lidé MeSH
- radioaktivní datování metody MeSH
- radioizotopy uhlíku analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Decellularized human pericardium is under study as an allogenic material for cardiovascular applications. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined with a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined by multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin was evaluated by means of an MTS assay. The viability of the cells, measured by their metabolic activity, decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a method by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged.
- MeSH
- alografty MeSH
- biokompatibilní materiály * chemie MeSH
- biomechanika MeSH
- endoteliální buňky pupečníkové žíly (lidské) cytologie metabolismus MeSH
- fibrin MeSH
- fibronektiny MeSH
- glutaraldehyd MeSH
- heterografty MeSH
- iridoidy MeSH
- kolagen chemie ultrastruktura MeSH
- lidé MeSH
- mikroskopie fluorescenční multifotonová MeSH
- perikard chemie transplantace ultrastruktura MeSH
- pevnost v tahu MeSH
- povrchová plasmonová rezonance MeSH
- reagencia zkříženě vázaná MeSH
- testování materiálů MeSH
- viabilita buněk MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
