Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae accounts for serious economic losses in the pig farming industry worldwide. We examined here the immunogenicity and protective efficacy of the recombinant type IV fimbrial subunit protein ApfA as a single antigen vaccine against pleuropneumonia, or as a component of a multi-antigen preparation comprising five other recombinant antigens derived from key virulence factors of A. pleuropneumoniae (ApxIA, ApxIIA, ApxIIIA, ApxIVA and TbpB). Immunization of pigs with recombinant ApfA alone induced high levels of specific serum antibodies and provided partial protection against challenge with the heterologous A. pleuropneumoniae serotype 9 strain. This protection was higher than that engendered by vaccination with rApxIVA or rTbpB alone and similar to that observed after immunization with the tri-antigen combination of rApxIA, rApxIIA and rApxIIIA. In addition, rApfA improved the vaccination potential of the penta-antigen mixture of rApxIA, rApxIIA, rApxIIIA, rApxIVA and rTbpB proteins, where the hexa-antigen vaccine containing rApfA conferred a high level of protection on pigs against the disease. Moreover, when rApfA was used for vaccination alone or in combination with other antigens, such immunization reduced the number of pigs colonized with the challenge strain. These results indicate that ApfA could be a valuable component of an efficient subunit vaccine for the prevention of porcine pleuropneumonia.

• MeSH
• Actinobacillus pleuropneumoniae genetika   imunologie   patogenita   MeSH
• bakteriální adheziny genetika   imunologie   MeSH
• bakteriální fimbrie genetika   imunologie   MeSH
• bakteriální vakcíny genetika   imunologie   MeSH
• Escherichia coli genetika   MeSH
• exotoxiny genetika   metabolismus   MeSH
• faktory virulence genetika   imunologie   MeSH
• infekce bakteriemi rodu Actinobacillus imunologie   prevence a kontrola   veterinární   MeSH
• molekulární sekvence - údaje MeSH
• nemoci prasat imunologie   prevence a kontrola   MeSH
• pleuropneumonie imunologie   prevence a kontrola   veterinární   MeSH
• polymerázová řetězová reakce veterinární   MeSH
• prasata MeSH
• rekombinantní proteiny genetika   imunologie   MeSH
• sekvenční analýza DNA veterinární   MeSH
• vakcinace veterinární   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH