BACKGROUND: Capnocytophaga canimorsus is a commensal bacterium found in the saliva of dogs and cats. Clinically significant infections in humans after a bite are often associated with the presence of immune deficiency. Early recognition and appropriate treatment are crucial for patient survival. In addition, patients with immune deficiency are susceptible to serious life-threatening nosocomial infections, which may also influence the prognosis of patients with Capnocytophaga canimorsus infection. CASE PRESENTATION: A 62-year-old Caucasian female was admitted with septic shock, acute respiratory distress syndrome, acute renal failure, metabolic acidosis and disseminated intravascular coagulation after suffering two small bites from her dog. She had received a splenectomy during childhood. The patient survived after early empiric treatment with antibiotics and intensive supportive care, including ventilation support, a high dose of noradrenalin, and continuous venovenous hemodialysis applied prior to the definitive diagnosis of Capnocytophaga canimorsus sepsis. She improved within 2 weeks but, despite all efforts to prevent nosocomial infection, her hospital course was complicated by Enterococcus species and Candida albicans pleuropneumonia that prolonged her stay in the intensive care unit, and necessitated ventilation support for 2 months. CONCLUSION: Severe Capnocytophaga canimorsus sepsis may be complicated by life-threatening nosocomial infection in immunocompromized patients. The prophylactic application of antibiotics after a dog bite should be considered in high-risk individuals with immune deficiency in order to prevent both Capnocytophyga canimorsus sepsis and serious nosocomial complications.

• MeSH
• akutní poškození ledvin komplikace   farmakoterapie   imunologie   mikrobiologie   MeSH
• antibakteriální látky terapeutické užití   MeSH
• Capnocytophaga imunologie   MeSH
• diseminovaná intravaskulární koagulace komplikace   farmakoterapie   imunologie   mikrobiologie   MeSH
• hostitel s imunodeficiencí * MeSH
• kousnutí a bodnutí komplikace   farmakoterapie   imunologie   mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• pleuropneumonie farmakoterapie   imunologie   mikrobiologie   patologie   MeSH
• psi MeSH
• septický šok komplikace   farmakoterapie   imunologie   mikrobiologie   MeSH
• syndrom dechové tísně komplikace   farmakoterapie   imunologie   mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae accounts for serious economic losses in the pig farming industry worldwide. We examined here the immunogenicity and protective efficacy of the recombinant type IV fimbrial subunit protein ApfA as a single antigen vaccine against pleuropneumonia, or as a component of a multi-antigen preparation comprising five other recombinant antigens derived from key virulence factors of A. pleuropneumoniae (ApxIA, ApxIIA, ApxIIIA, ApxIVA and TbpB). Immunization of pigs with recombinant ApfA alone induced high levels of specific serum antibodies and provided partial protection against challenge with the heterologous A. pleuropneumoniae serotype 9 strain. This protection was higher than that engendered by vaccination with rApxIVA or rTbpB alone and similar to that observed after immunization with the tri-antigen combination of rApxIA, rApxIIA and rApxIIIA. In addition, rApfA improved the vaccination potential of the penta-antigen mixture of rApxIA, rApxIIA, rApxIIIA, rApxIVA and rTbpB proteins, where the hexa-antigen vaccine containing rApfA conferred a high level of protection on pigs against the disease. Moreover, when rApfA was used for vaccination alone or in combination with other antigens, such immunization reduced the number of pigs colonized with the challenge strain. These results indicate that ApfA could be a valuable component of an efficient subunit vaccine for the prevention of porcine pleuropneumonia.

• MeSH
• Actinobacillus pleuropneumoniae genetika   imunologie   patogenita   MeSH
• bakteriální adheziny genetika   imunologie   MeSH
• bakteriální fimbrie genetika   imunologie   MeSH
• bakteriální vakcíny genetika   imunologie   MeSH
• Escherichia coli genetika   MeSH
• exotoxiny genetika   metabolismus   MeSH
• faktory virulence genetika   imunologie   MeSH
• infekce bakteriemi rodu Actinobacillus imunologie   prevence a kontrola   veterinární   MeSH
• molekulární sekvence - údaje MeSH
• nemoci prasat imunologie   prevence a kontrola   MeSH
• pleuropneumonie imunologie   prevence a kontrola   veterinární   MeSH
• polymerázová řetězová reakce veterinární   MeSH
• prasata MeSH
• rekombinantní proteiny genetika   imunologie   MeSH
• sekvenční analýza DNA veterinární   MeSH
• vakcinace veterinární   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH