- MeSH
- balneologie metody trendy MeSH
- duševní poruchy * metabolismus rehabilitace MeSH
- hydrokortison krev metabolismus MeSH
- kortikosteron krev metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- limbický systém metabolismus MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- pregnenolon krev metabolismus MeSH
- sebeposuzující dotazníky PHQ MeSH
- serotonin krev metabolismus MeSH
- techniky cvičení a pohybu MeSH
- terapie cvičením MeSH
- tryptofan krev metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
The trichothecene mycotoxins contaminate cereal grains and have been related to alimentary toxicosis resulted in emetic response. This family of mycotoxins comprises type A to D groups of toxic sesquiterpene chemicals. Diacetoxyscirpenol (DAS), one of the most toxic type A trichothecenes, is considered to be a potential risk for human and animal health by the European Food Safety Authority. Other type A trichothecenes, T-2 toxin and HT-2 toxin, as well as type B trichothecene deoxynivalenol (DON), have been previously demonstrated to induce emetic response in the mink, and this response has been associated with the plasma elevation of neurotransmitters peptide YY (PYY) and serotonin (5-hydroxytryptamine, 5-HT). However, it is found that not all the type A and type B trichothecenes have the capacity to induce PYY and 5-HT. It is necessary to identify the roles of these two emetogenic mediators on DAS-induced emesis. The goal of this study was to determine the emetic effect of DAS and relate this effect to PYY and 5-HT, using a mink bioassay. Briefly, minks were fasted one day before experiment and given DAS by intraperitoneally and orally dosing on the experiment day. Then, emetic episodes were calculated and blood collection was employed for PYY and 5-HT test. DAS elicited robust emetic responses that corresponded to upraised PYY and 5-HT. Blocking the neuropeptide Y2 receptor (NPY2R) diminished emesis induction by PYY and DAS. The serotonin 3 receptor (5-HT3R) inhibitor granisetron totally restrained the induction of emesis by serotonin and DAS. In conclusion, our findings demonstrate that PYY and 5-HT have critical roles in DAS-induced emetic response.
- MeSH
- antiemetika farmakologie MeSH
- granisetron farmakologie MeSH
- modely nemocí na zvířatech MeSH
- norek MeSH
- peptid YY krev MeSH
- receptory gastrointestinálních hormonů metabolismus MeSH
- receptory serotoninové 5-HT3 metabolismus MeSH
- sekreční dráha MeSH
- serotonin krev MeSH
- serotoninové receptory 5-HT3 - antagonisté farmakologie MeSH
- trichotheceny * MeSH
- upregulace MeSH
- zvířata MeSH
- zvracení krev chemicky indukované prevence a kontrola MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: During blood feeding, sand flies inoculate salivary proteins that interact with the host haemostatic system. The blocking of biogenic amines such as serotonin and histamine helps to limit vasodilatation and clot formation, and thus enables the insect to finish the blood-feeding process. In sand flies, an amine-binding ability is known only for the yellow-related proteins of Phlebotomus and Lutzomyia vectors, but not yet for members of the genus Sergentomyia. METHODS: The ability of Phlebotomus argentipes and Sergentomyia schwetzi recombinant yellow-related salivary proteins to bind histamine and serotonin was measured by microscale thermophoresis. Both sand fly species were also fed through a chicken-skin membrane on blood mixed with histamine or serotonin in order to check the effects of biogenic amines on sand fly fitness. Additionally, fecundity and mortality were compared in two groups of P. argentipes females fed on repeatedly-bitten and naive hamsters, respectively. RESULTS: The P. argentipes recombinant yellow-related protein PagSP04 showed high binding affinity to serotonin and low affinity to histamine. No binding activity was detected for two yellow-related proteins of S. schwetzi. Elevated concentrations of serotonin significantly reduced the amount of eggs laid by P. argentipes when compared to the control. The fecundity of S. schwetzi and the mortality of both sand fly species were not impaired after the experimental membrane feeding. Additionally, there were no differences in oviposition or mortality between P. argentipes females fed on immunized or naive hamsters. CONCLUSIONS: Our results suggest that in natural conditions sand flies are able to cope with biogenic amines or anti-saliva antibodies without any influence on their fitness. The serotonin binding by salivary yellow-related proteins may play an important role in Phlebotomus species feeding on mammalian hosts, but not in S. schwetzi, which is adapted to reptiles.
- MeSH
- biogenní aminy * krev farmakologie MeSH
- fertilita účinky léků MeSH
- histamin krev MeSH
- hmyzí proteiny chemie metabolismus MeSH
- kousnutí a bodnutí hmyzem imunologie MeSH
- křečci praví MeSH
- krev metabolismus MeSH
- molekulární evoluce MeSH
- mortalita MeSH
- Phlebotomus metabolismus MeSH
- plazi MeSH
- protilátky MeSH
- Psychodidae metabolismus MeSH
- rekombinantní proteiny chemie metabolismus MeSH
- savci MeSH
- serotonin krev MeSH
- slinné proteiny a peptidy * chemie metabolismus MeSH
- sliny imunologie MeSH
- vazba proteinů MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Za účelem objektivizace změn hladin metabolomu neurosteroidů u dospělých klientů po komplexní lázeňské léčebně rehabilitační péči navázaly v roce 2017 vzájemnou spolupráci Endokrinologický ústav v Praze a Priessnitzovy léčebné lázně a.s. v Jeseníku. Autoři prezentují soubor 70 probandů, kteří absolvovali při nástupu a dimisi baterii klinických a laboratorních vyšetření. První výsledky změn metabolomu neurosteroidů, kineziologického vyšetření diagnostickou části Computer Kinesiology a výsledky Knoblochova dotazníku sebeposuzovací škály N-5 ukazují jednoznačně efekt léčení v lázních.
The Endocrinological Institute in Prague established the cooperation with in Priessnitz's spa a.s. in Jesenik intention to objectify changes in neurosteroid metabolomic biomarkers levels in adult clients after spa treatment, in 2017. The authors present a set of 70 probands. The first results of changes in the levels of neurosteroids, serotonin and homocysteine metabolite clearly demonstrate the effect of treatment in the spa. The kinesiological examination of the diagnostic part of Computer Kinesiology and the numeric outputs of Knobloch questionnaire (N-5 self- -judging scale) correlate with endocrinological results.
- Klíčová slova
- Knoblochův dotazník N-5,
- MeSH
- balneologie * MeSH
- dospělí MeSH
- hodnocení výsledků péče pacientem MeSH
- hodnocení výsledků zdravotní péče MeSH
- homocystein analýza krev MeSH
- kineziologie aplikovaná metody MeSH
- klinická studie jako téma MeSH
- klinické laboratorní techniky metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- neurotransmiterové látky * analýza krev metabolismus MeSH
- průzkumy a dotazníky MeSH
- senioři MeSH
- serotonin analýza krev MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- práce podpořená grantem MeSH
- MeSH
- akinetický mutismus MeSH
- analgetika škodlivé účinky MeSH
- antidepresiva škodlivé účinky MeSH
- biochemická analýza krve MeSH
- časná diagnóza MeSH
- diferenciální diagnóza MeSH
- lékové interakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- pneumonie MeSH
- prognóza MeSH
- serotonin krev MeSH
- serotoninový syndrom * diagnóza etiologie terapie MeSH
- vorikonazol terapeutické užití MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
The aim of this study was to examine the effect of alterations in potential lead fall distance on the hormonal responses of rock climbers. Nine advanced female climbers completed two routes while clipping all (PRO-all) or half (PRO-½) of the fixed points of protection. Venous blood samples were analysed for total catecholamines, noradrenaline (norepinephrine), adrenaline (epinephrine), dopamine, lactate, cortisol and serotonin. Differences between the two conditions pre, immediately post and 15 min post climbing were assessed using a 2 × 3 repeated measures ANOVA. All hormones and blood lactate concentrations increased significantly (P < 0.05) immediately post climb, except for cortisol. Peak cortisol concentrations did not occur until 15 min post ascent. Further, significant interactions between climbing and clipping conditions were found for total catecholamines (890% of basal concentration in PRO-½ vs. 568% in PRO-all), noradrenaline (794% vs. 532%) and dopamine (500% vs. 210%). There were no significant interactions for adrenaline (1920% vs. 1045%), serotonin (150% vs. 127%) or lactate (329% vs. 279%). The study showed a greater catecholamine response with an increase in potential lead fall distance. The most pronounced increases seen in catecholamine concentration were reported for dopamine and noradrenaline.
- MeSH
- adrenalin krev MeSH
- dopamin krev MeSH
- dospělí MeSH
- hormony krev MeSH
- horolezectví zranění fyziologie psychologie MeSH
- hydrokortison krev MeSH
- kyselina mléčná krev MeSH
- lidé MeSH
- noradrenalin krev MeSH
- odpočinek MeSH
- percepce MeSH
- serotonin krev MeSH
- srdeční frekvence fyziologie MeSH
- strach MeSH
- tělesná námaha fyziologie MeSH
- úzkost * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.
- MeSH
- fosfopyruváthydratasa krev metabolismus MeSH
- hmotnostní spektrometrie MeSH
- kolorektální nádory krev diagnóza MeSH
- lidé středního věku MeSH
- lidé MeSH
- metabolomika MeSH
- proteiny 14-3-3 krev metabolismus MeSH
- proteomika MeSH
- pyruvátkinasa krev metabolismus MeSH
- senioři MeSH
- serotonin krev metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Impaired daily rhythms in vertebrate physiology occur with age. Particularly, age-related changes in melatonin and serotonin rhythms and hypercortisolemia have been reported to be linked to age-related disorders. This study was aimed at assessing the effect of a Jerte Valley cherry-based nutraceutical product (patent no ES 2342141 B1), which contains high levels of tryptophan, serotonin, and melatonin, on the serum melatonin, serotonin, corticosterone, and total antioxidant capacity (TAC) levels in young and old ring doves (Streptopelia risoria) and rats (Rattus norvegicus) as representatives of animals with diurnal and nocturnal habits, respectively. The animals consumed the cherry product for 10 days. Serum melatonin, serotonin, corticosterone, and TAC were measured with commercial ELISA kits. The consumption of the cherry product induced a significant increase in the circulating levels of melatonin and serotonin, as well as in the serum TAC and a significant decrease in the circulating levels of corticosterone in both species and groups of age as compared to their respective values in the control groups. The consumption of a Jerte Valley cherry-based nutraceutical product may help to counteract the decrease in melatonin and serotonin and the increase in oxidative stress, suggesting a potential health benefit especially in aged populations where these parameters have been found to be altered.
- MeSH
- antioxidancia MeSH
- cirkadiánní rytmus fyziologie genetika účinky léků MeSH
- Columbidae MeSH
- ELISA metody využití MeSH
- experimenty na zvířatech MeSH
- financování organizované MeSH
- kortikosteron krev MeSH
- Malpighiaceae MeSH
- melatonin izolace a purifikace krev MeSH
- potkani Wistar MeSH
- serotonin izolace a purifikace krev MeSH
- stárnutí MeSH
- statistika jako téma MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
Zácpa je častý syndrom postihující miliony lidí - syndrom, který se brání definitivní léčbě, způsobuje miliony ročních návštěv lékařů, za léky bez lékařského předpisu se za ni utratí miliony dolarů ročně, je provázena ekonomickými následky pro komunitu i jedince a sníženou kvalitou života. Hypotéza: Může vhodná aplikace wellness a změny životního stylu snížit riziko vzniku zácpy, změnit již přítomnou zácpu a zlepšit kvalitu života? Metoda: Vyšetřili jsme problematiku zácpy z hlediska veřejného zdraví ve 21. století. Prohlédli jsme recentní literaturu o zácpě z hlediska epidemiologie, statistiky, ekonomických následků, geografické polohy, pohlaví, socioekonomické situace, úrovně vzdělání a zdravotnické osvěty, stravovacích návyků, fyzické aktivity, úrovně stresu, komorbidity, užívaných léků a základních přírodních principů. Dva recenzenti provedli hledání citací ve Scholar Google a na Medline. Výsledky: Vyvinuli jsme teoretický model zácpy - problém veřejného zdraví ve 21. století z hlediska rizikových faktorů, prevence, léčby a aplikace medicíny wellness/změna životního stylu (tab. 1). Závěry: Zácpu je třeba zkoumat z různých hledisek. Pro prevenci a léčbu zácpy doporučujeme nový styl wellness/změnu životního stylu. Je nezbytné, aby poskytovatelé léčebné péče začali aplikovat tento postoj do svého lékařského modelu léčby. Také navrhujeme, aby zácpa nebyla považována za samostatný syndrom, ale jako první signál potenciálního porušení biochemické tělesné rovnováhy, který může vést k vývoji chronických onemocnění.
Constipation is a common syndrome, afflicting millions - that has so far defied a definitive cure, causing millions of physician visits per year, spending millions of dollars on over the counter medication, having economic consequences to the community/individuals and decreasing the quality of life. Hypothesis: Can a proper application of wellness/lifestyle medicine decrease the risk of development of constipation, reverse the already establish constipation and improve the quality of life? Methods: We investigated the public health problem with constipation in 21st century. We reviewed the recent literature on constipation from the perspectives of epidemiology, statistics, economic consequences, SES (social economic status), health literacy and education level, geographic area, gender, eating behavior, physical activity, stress level, co-morbidities, medication use and basic principles of the nature. Two reviewers performed reference search on Scholar Google and PubMed. Results: We developed a theoretical model of constipation -the public health problem in the 21st century from the perspectives of risk factors, prevention, treatment and application of wellness/lifestyle medicine (tab. 1). Conclusion: Constipation needs to be seen from completely different perspectives. We propose a new wellness/lifestyle medicine approach for prevention and treatment of constipation. It is necessary that health care providers will start to apply this approach into their medical model of treatment. We also suggest that constipation should not be seen as an isolated symptom, but as a first signal of potential biochemical imbalances in the body, which can lead to development of chronic diseases.
- Klíčová slova
- wellness, výživa, fyzická aktivita, stres,
- MeSH
- komorbidita MeSH
- lidé MeSH
- preference v jídle MeSH
- psychický stres komplikace MeSH
- rizikové faktory MeSH
- serotonin krev nedostatek MeSH
- zácpa epidemiologie etiologie terapie MeSH
- životní styl MeSH
- Check Tag
- lidé MeSH
