Low-temperature plasma Dotaz Zobrazit nápovědu
Nízkoteplotní plazma produkované stejnosměrnými elektrickými výboji se jeví jako vhodný prostředek inaktivace plísní. Tato teze je dokumentována na přehledu výsledků dosažených studiemi in vitro i in vivo. Fungi jsou sice na působení plazmatu méně citlivé než bakterie a citlivost jejich jednotlivých druhů je různá, původci dermatomykóz jsou však inaktivováni expozicemi do 25 minut. Na arteficiálně infikovaných morčatech bylo prokázáno jak zkrácení doby onemocnění, tak i snížení počtu zárodků infekčního agens. Použití plazmatu v dermatologii je dokumentováno i na případu z humánní medicíny. Pro expozici pokožky plazmatu nebyly zjištěny žádné nepříznivé vedlejší účinky.
Low temperature plasma produced by direct current electric discharges seems to be a useful tool for inactivation of fungi. This thesis is documented in this review of previous results of in vitro and in vivo studies. Fungi are less sensitive to plasma exposure than bacteria and their sensitivity differs among strains, causative agents of dermatomycoses, however, are inactivated within 25 minutes. Both shortening disease and reducing the number of germs was proved on artificially infected guinea pigs. The use of plasma in dermatology is also documented on a case of human medicine. The exposure of skin by plasma exhibits no adverse side effects.
- MeSH
- dermatomykózy * terapie MeSH
- experimenty na zvířatech MeSH
- lidé MeSH
- mladý dospělý MeSH
- morčata MeSH
- plazmové plyny * aplikace a dávkování terapeutické užití MeSH
- počet mikrobiálních kolonií MeSH
- techniky in vitro MeSH
- tinea terapie MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mladý dospělý MeSH
- morčata MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- kazuistiky MeSH
- práce podpořená grantem MeSH
A case of dermatomycosis caused by zoophilic strain of Trichophyton interdigitale was treated by low-temperature plasma produced by direct current (DC) cometary discharge. The shortening of skin lesion persistence along with suppression of subjective discomfort and etiological agent was observed.
The fungicidal effect of low-temperature plasma generated by positive direct current discharge and its influence on the growth dynamics was evaluated on three micromycete species and yeast in water suspensions. The fungicidal effect was lower than analogous bactericidal effect and differs substantially among various fungal species. Together with the cidal effects, the slower growth of exposed fungal spores was observed.
The inactivation of Schistosoma mansoni cercariae and miracidia was achieved by exposure to plasma produced by the positive, negative, and axial negative corona discharges. The positive discharge appeared as the most effective, causing the death of cercariae and miracidia within 2-3 min of exposure. The negative discharge was less effective, and the axial discharge was ineffective. The water pre-activated (PAW) by the discharges showed similar efficiency, with the exception of the significantly effective PAW activated with axial discharge. These facts, together with the observation of various reactions among plasma-damaged schistosomes, suggest that the mechanisms of inactivation by different types of discharges are different.
- Publikační typ
- časopisecké články MeSH
An advanced surface engineering process combining micro-texture with a plasma carburising process was produced on CoCrMo femoral head, and their tribological properties were evaluated by the cutting-edge pendulum hip joint simulator coupled with thin film colorimetric interferometry. FESEM and GDOES showed that precipitation-free C S-phase with a uniform case depth of 10μm was formed across the micro-textures after duplex treatment. Hip simulator tests showed that the friction coefficient was reduced by 20% for micro-metre sized texture, and the long-term tribological property of microtexture was enhanced by the C-supersaturated crystalline microstructure formed on the surface of duplex treated CoCrMo, thereby enhancing biotribological durability significantly. In-situ colorimetric interferometry confirmed that the maximum film thickness around texture area was 530nm, indicating that the additional lubricant during sliding motion might provide exceptional bearing life.
- MeSH
- atmosférický tlak MeSH
- Bacteria účinky záření MeSH
- biofyzika záření metody přístrojové vybavení trendy MeSH
- dekontaminace metody normy přístrojové vybavení MeSH
- finanční podpora výzkumu jako téma MeSH
- financování organizované MeSH
- lidé MeSH
- reaktivní formy kyslíku chemie škodlivé účinky účinky záření MeSH
- singletový kyslík škodlivé účinky účinky záření MeSH
- sterilizace metody přístrojové vybavení využití MeSH
- ultrafialové záření škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- MeSH
- atmosférický tlak MeSH
- Bacteria účinky záření MeSH
- biofyzika záření metody přístrojové vybavení trendy MeSH
- dekontaminace metody normy přístrojové vybavení MeSH
- finanční podpora výzkumu jako téma MeSH
- financování organizované MeSH
- lidé MeSH
- reaktivní formy kyslíku chemie škodlivé účinky účinky záření MeSH
- singletový kyslík škodlivé účinky účinky záření MeSH
- sterilizace metody přístrojové vybavení využití MeSH
- ultrafialové záření škodlivé účinky MeSH
- Check Tag
- lidé MeSH
By definition, the nonthermal plasma (NTP) is partially ionized gas where the energy is stored mostly in the free electrons and the overall temperature remains low. NTP is widely used for many years in various applications such as low-temperature plasma chemistry, removal of gaseous pollutants, in gas-discharge lamps or surface modification. However, during the last ten years, NTP usage expanded to new biological areas of application like plasma microorganisms' inactivation, ready-to-eat food preparation, biofilm degradation or in healthcare, where it seems to be important for the treatment of cancer cells and in the initiation of apoptosis, prion inactivation, prevention of nosocomial infections or in the therapy of infected wounds. These areas are presented and documented in this paper as a review of representative publications.
- MeSH
- čištění vody MeSH
- dekontaminace * MeSH
- dezinfekce * MeSH
- lidé MeSH
- nádory terapie MeSH
- obaly potravin MeSH
- plazmové plyny farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Steroids constitute one of the most important groups of compounds of regulatory properties both in the animal and plant kingdom. In plants, steroids such as brassinosteroids or progesterone, by binding to protein receptors in cell membranes, regulate growth and initiate processes leading to increased tolerance to stress conditions. Due to their structural similarities to sterols, these steroids may also directly interact with cellular membranes. Our aim was to determine the changes of the structural parameters of lipid membranes under the influence of hydrophobic steroid compounds, i.e., 24-epibrassinolide (EBR) and its precursor-24-epicastasterone (ECS) and progesterone (PRO). Lipids were isolated from wheat seedlings with different tolerances to frost, grown at low temperatures (5 °C) for 1.5 and 3 weeks (acclimation process). Control plants were cultured continuously at 20 °C. From galactolipids and phospholipids, the main polar lipid fractions, the monolayers were formed, using a technique of Langmuir trough. EBR and ECS were introduced into monolayers, together with lipids, whereas the PRO was dissolved in the aqueous sub-phase upon which the monolayers were spread. Measurements performed at 25 °C and 10 °C showed a significant action of the tested compounds on the physicochemical properties of the monolayers. EBR and PRO increased the area per lipid molecule in monolayers, resulting in formation of more flexible surface structures while the presence of the ECS induced the opposite effect. The influence of the polarity of lipids and steroids on the interactions in the monolayer was discussed. Lipids extracted from the membranes of wheat with the most tolerance to frost were characterized by the highest fatty acid unsaturation and steroids had a relatively weak effect on the parameters of the structure of their monolayers.
- MeSH
- brassinosteroidy chemie metabolismus farmakologie MeSH
- buněčná membrána chemie účinky léků MeSH
- membránové lipidy chemie metabolismus MeSH
- metabolismus lipidů účinky léků MeSH
- nízká teplota MeSH
- progesteron chemie metabolismus farmakologie MeSH
- pšenice účinky léků růst a vývoj MeSH
- semenáček účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
