The phenolic compounds of methanolic extracts of Salvia pomifera and Salvia fruticosa were identified by liquid chromatography tandem mass spectrometry. Carnosic acid and its metabolite carnosol were the most abundant terpene phenolic compounds of S. fruticosa, while they were completely absent in S. pomifera. The main terpene phenolic constituent of S. pomifera was 12-O-methylcarnosic acid and its mass/mass fragmentation pathway was explained. The detailed mechanism of carnosic acid oxidation to carnosol was suggested. The effects of Salvia extracts and/or carnosic acid, the main diterpene phenolic component of S. fruticosa, on the proliferation and cell cycle of two melanoma cell lines (A375, Mel JuSo) and human fibroblast cell line (HFF) were investigated by MTT assay, PI-exclusion assay and flow cytometry cell cycle analysis. Extract of S. fruticosa more efficiently than S. pomifera extract reduced the proliferation of the human melanoma cells. Carnosic acid showed the most significant effect. The first evidence that carnosic acid affects microtubule dynamics and arrests the cell cycle in the G2/M phase was provided. Collectively, our results demonstrate that these two Salvia species are plants of medicinal interest with perspective for further investigation. Carnosic acid could be the compound responsible for the biological activities of S. fruticosa extracts.
- Klíčová slova
- 12-O-methylcarnosic acid, LC-MS, Salvia fruticosa, Salvia pomifera, cancer, carnosic acid, cell cycle, cytotoxicity, melanoma, microtubules,
- MeSH
- antitumorózní látky chemie izolace a purifikace farmakologie MeSH
- buněčné linie MeSH
- diterpeny abietanové chemie izolace a purifikace farmakologie MeSH
- epitelové buňky účinky léků patologie MeSH
- fenoly chemie izolace a purifikace farmakologie MeSH
- fibroblasty cytologie účinky léků MeSH
- inhibiční koncentrace 50 MeSH
- kontrolní body fáze G2 buněčného cyklu účinky léků MeSH
- lidé MeSH
- methanol chemie MeSH
- nádorové buněčné linie MeSH
- nadzemní části rostlin chemie MeSH
- oxidace-redukce MeSH
- proliferace buněk účinky léků MeSH
- rostlinné extrakty chemie MeSH
- rozpouštědla chemie MeSH
- šalvěj chemie MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antitumorózní látky MeSH
- carnosol MeSH Prohlížeč
- diterpeny abietanové MeSH
- fenoly MeSH
- methanol MeSH
- rostlinné extrakty MeSH
- rozpouštědla MeSH
- salvin MeSH Prohlížeč
