Monoclonal antibodies IAH-CC51, BB6-11C9.6 and B-Ly4 are routinely used to detect CD21 orthologue on the surface of porcine B lymphocytes. Cross-reactive studies show that IAH-CC51 and B-Ly4 recognize only a portion of B cells that are positive for pan-specific BB6-11C9.6. This indicates that CD21 is always present on all mature B cells but can be expressed in at least two differential forms, and these were assigned as CD21(a) and CD21(b). We used IAH-CC51 together with anti-CD2 to define four subpopulations of B cells. Ontogenetic and in vitro culture studies, analysis of cell size, expression of CD11b and class-switched phenotype together with measurement of proliferation and cell death, revealed that these subsets represent distinct populations. Phenotypic and functional features collectively suggest that CD21(b+) B cells are less mature than CD21(b-). The present work is the first to show that distinct subsets of mature B cells can express differential forms of CD21.

• MeSH
• apoptóza MeSH
• epitopy imunologie   MeSH
• kultivované buňky MeSH
• monoklonální protilátky imunologie   MeSH
• podskupiny B-lymfocytů klasifikace   imunologie   MeSH
• prasata imunologie   MeSH
• receptory komplementu 3d imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• epitopy MeSH
• monoklonální protilátky MeSH
• receptory komplementu 3d MeSH

The expression of selected molecules was chosen to study porcine γδ lymphocytes and their CD2/CD8 subsets in different lymphoid organs in vivo and in vitro. Results indicate that many γδ T cells can constitutively express CD25 and MHC-II and that the frequency of γδ T cells positive for CD25, CD11b, SWC1 and SWC7 can be increased by stimulation. A diversified TCRδ repertoire was found inside CD25(+), CD11b(+), SWC1(-) and CD45RA(-) cells. Ontogenetic studies revealed various age and/or colonization dependency for expression of all studied molecules except of SWC7. Findings generally indicate that CD25 represent an activation molecule that probably marks a functionally distinct subsets, expression of CD11b is perhaps connected to early functions of naive γδ T cells in the periphery, SWC1 is lineage specific marker, SWC7 may represent an activation molecule with intrinsic or transient expression, and the expression of CD45RA/RC most likely defines naive and terminally differentiated cells.

• MeSH
• antigeny CD45 metabolismus   MeSH
• CD antigeny metabolismus   MeSH
• prasata imunologie   MeSH
• receptor interleukinu-2 - alfa-podjednotka metabolismus   MeSH
• T-lymfocyty - podskupiny cytologie   imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD45 MeSH
• CD antigeny MeSH
• receptor interleukinu-2 - alfa-podjednotka MeSH

Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the development of different subpopulations of alphabeta T, gammadelta T and B lymphocytes as well as development of innate immunity and the acquisition of full immunological capacities are discussed here for swine and compared with other species. The description of related topics such as fertilization, morphogenesis, maternal-fetal-neonatal physiology and early neonatal development are also discussed.

• MeSH
• B-lymfocyty cytologie   imunologie   metabolismus   MeSH
• buněčná imunita MeSH
• embryo savčí imunologie   MeSH
• hematopoetické kmenové buňky cytologie   imunologie   MeSH
• hematopoéza imunologie   MeSH
• imunitní systém embryologie   imunologie   MeSH
• lymfopoéza MeSH
• maternofetální výměna látek imunologie   MeSH
• morfogeneze imunologie   MeSH
• placentace imunologie   MeSH
• prasata embryologie   imunologie   virologie   MeSH
• přirozená imunita MeSH
• T-lymfocyty cytologie   imunologie   metabolismus   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH