Interindividual differences in DNA repair systems may play a role in modulating the individual risk of developing colorectal cancer. To better ascertain the role of DNA repair gene polymorphisms on colon and rectal cancer risk individually, we evaluated 15,419 single nucleotide polymorphisms (SNPs) within 185 DNA repair genes using GWAS data from the Colon Cancer Family Registry (CCFR) and the Genetics and Epidemiology of Colorectal Cancer Consortium (GECCO), which included 8,178 colon cancer, 2,936 rectum cancer cases and 14,659 controls. Rs1800734 (in MLH1 gene) was associated with colon cancer risk (p-value = 3.5 × 10-6 ) and rs2189517 (in RAD51B) with rectal cancer risk (p-value = 5.7 × 10-6 ). The results had statistical significance close to the Bonferroni corrected p-value of 5.8 × 10-6 . Ninety-four SNPs were significantly associated with colorectal cancer risk after Binomial Sequential Goodness of Fit (BSGoF) procedure and confirmed the relevance of DNA mismatch repair (MMR) and homologous recombination pathways for colon and rectum cancer, respectively. Defects in MMR genes are known to be crucial for familial form of colorectal cancer but our findings suggest that specific genetic variations in MLH1 are important also in the individual predisposition to sporadic colon cancer. Other SNPs associated with the risk of colon cancer (e.g., rs16906252 in MGMT) were found to affect mRNA expression levels in colon transverse and therefore working as possible cis-eQTL suggesting possible mechanisms of carcinogenesis.
- Klíčová slova
- DNA repair, cancer susceptibility, colon cancer, genome-wide association studies, rectal cancer, single nucleotide polymorphisms,
- MeSH
- biologická variabilita populace genetika MeSH
- DNA modifikační methylasy genetika MeSH
- DNA vazebné proteiny genetika MeSH
- dospělí MeSH
- enzymy opravy DNA genetika MeSH
- genetická predispozice k nemoci * MeSH
- hodnocení rizik MeSH
- jednonukleotidový polymorfismus MeSH
- karcinogeneze genetika MeSH
- kolon patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- MutL homolog 1 genetika MeSH
- nádorové supresorové proteiny genetika MeSH
- nádory rekta genetika patologie MeSH
- nádory tračníku genetika patologie MeSH
- oprava DNA genetika MeSH
- registrace statistika a číselné údaje MeSH
- rektum patologie MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- DNA modifikační methylasy MeSH
- DNA vazebné proteiny MeSH
- enzymy opravy DNA MeSH
- MGMT protein, human MeSH Prohlížeč
- MLH1 protein, human MeSH Prohlížeč
- MutL homolog 1 MeSH
- nádorové supresorové proteiny MeSH
- RAD51B protein, human MeSH Prohlížeč
