AIM: To determine the occurrence of species of Babesia potentially pathogenic for humans in ticks and in the blood of dogs and deer in selected regions of the Czech Republic. To compare the prevalence of Babesia spp. in ticks with that of other tick-borne pathogens, such as Borrelia spp., Anaplasma spp., and Rickettsia spp. MATERIAL AND METHODS: Tick samples were individually homogenized. DNA was isolated from tick samples and animal blood. The detection of Babesia spp. was based on PCR of the 18S rRNA gene, and the identification to the species level was done by sequencing analysis of the PCR products. RESULTS: In 2014-2016, ticks and blood of dogs and deer collected in various areas of the Czech Republic were analyzed. In a set of 675 Ixodes ricinus ticks, the positivity rate for Babesia spp. varied from 0.0 to 3.3 %. The species Babesia venatorum, Babesia microti (both pathogenic for humans), and Babesia capreoli were identified in ticks by sequencing analysis. The prevalence of Babesia spp. in ticks compared to that of other pathogens such as Borrelia burgdorferi s. l. (29.3 %) or Anaplasma phagocytophilum (4.9 %) was lower and comparable to that of Rickettsia spp. (1.6 %). Co-infection with Borrelia burgdorferi s.l (B. venatorum - Borrelia garinii, Borrelia afzelii, and B. microti - B. afzelii) was found in a third of Babesia spp. positive ticks. Out of 109 dog blood samples, 3.7 % were positive for Babesia spp., specifically Babesia gibsoni and Babesia vulpes. Of 50 blood samples of wild deer from the natural ecosystem, the positivity rate reached 4.0 %. The species Babesia divergens, a major human pathogen, was identified. Out of 80 blood samples from farmed deer, 5.0 % were positive for the species Babesia odocoilei. Nucleotide sequences of the agents causing human babesiosis were deposited in the gene bank under accession numbers ON892053 (B. venatorum), ON892061 (B. microti), and ON892067 (B. divergens). CONCLUSIONS: Using PCR of the 18S rRNA gene and amplicon sequencing, three species of Babesia causing human babesiosis were detected in the Czech Republic: B. divergens, B. venatorum, and B. microti. Babesia spp. pathogenic for humans pose a potential risk especially in asplenic and immunocompromised patients. The detected co-infections with Borrelia spp. can be the cause of a complicated course of the disease.

• Klíčová slova
• Anaplasma spp., Babesia spp., Borrelia burgdorferi s. l., Czech Republic, Deer, PCR, Rickettsia spp., Sequence analysis, co-infection, dog, tick,
• MeSH
• Babesia * izolace a purifikace   genetika   klasifikace   MeSH
• babezióza epidemiologie   parazitologie   MeSH
• klíště * mikrobiologie   parazitologie   MeSH
• psi MeSH
• RNA ribozomální 18S genetika   MeSH
• vysoká zvěř * parazitologie   MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• RNA ribozomální 18S MeSH

Ixodes ricinus ticks are considered as the vector of the Borrelia burgdorferi sensu lato complex in urban areas, including city parks and green recreational areas. The aim of the present study was to determine the prevalence of B. burgdorferi s. l. in urban areas in the city of Prague, Czech Republic. In selected public green areas in Prague, a total of 2819 I. ricinus ticks were collected in spring, from April to June, in 2014-2020. Quantitative real time PCR revealed 28.1% of ticks (31% of males, 33.7% of females and 25.8% of nymphs) to be positive for B. burgdorferi s. l. The prevalence varied significantly (p˂0.01) between collection sites, with the highest numbers of infected ticks found in the central city areas. The places serving people for recreational and sport activities in urban areas are characterized by a lower diversity of reservoir hosts, provide opportunity for exposure to Borrelia infected ticks, and pose a higher infection risk. We have detected seven Borrelia species in ticks: B. garinii, B. afzelii, B. bavariensis, B. burgdorferi sensu stricto, B. valaisiana, B. spielmanii, and B. finlandensis. Most positive ticks were infected by B. garinii (35%) and B. afzelii (36.9%). Our results show that the Borrelia transmission cycle occurs within urban biotops and highlight the need for surveillance of tick-borne pathogens in public green areas.

• Klíčová slova
• Borrelia, Ixodes ricinus, Tick, Tick-borne disease, Urban areas,
• Publikační typ
• časopisecké články MeSH

BACKGROUND: A general characteristic of cancer metabolism is the skill to gain the essential nutrients from a relatively poor environment and use them effectively to maintain viability and create new bio-mass. The changes in intracellular and extracellular metabolites that accompany metabolic reprogramming associated with tumor growth subsequently affect gene expression, cell differentiation, and tumor microenvironment. During carcinogenesis, cancer cells face huge selection pressures that force them to constantly optimize dominant metabolic pathways and undergo major metabolic reorganizations. In general, greater flexibility of metabolic pathways increases the ability of tumor cells to satisfy their metabolic needs in a changing environment. PURPOSE: In this review, we discuss the metabolic properties of cancer cells and describe the tumor promoting effect of the transformed metabolism. We assume that changes in metabolism are significant enough to facilitate tumorigenesis and may provide interesting targets for cancer therapy.

• Klíčová slova
• Krebs cycle, Metabolism, Warburg effect, anaplerosis, cancer, glutaminolysis, malignancy, oncogenesis, oncometabolite,
• MeSH
• karcinogeneze metabolismus   MeSH
• lidé MeSH
• metabolické sítě a dráhy MeSH
• nádorová transformace buněk metabolismus   MeSH
• nádorové mikroprostředí MeSH
• nádory * metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

The coexistence of species in a given community depends on the set of species involved and the timing of their interactions. Many native communities are increasingly forced to face both direct and indirect pressures from new alien predators, which, in extreme cases, can lead to the extinction of prey populations. In this study, we examine the dynamics of the ontogeny of common frog (Rana temporaria) tadpoles under different time patterns of an alien predator-the red-eared slider (Trachemys scripta elegans) presence. We found that the tadpoles had a longer larval period and were smaller in size at metamorphosis and lower in body mass when the predator was present in early development than when the tadpoles developed without a predator. The early presence of a predator conspicuously reduced the growth increments of the tadpoles at early development. After the removal of the predator, growth accelerated above the level measured under the conditions of both the late predator and no predator. However, these growth rates did not exceed the growth rates of equally sized tadpoles in the other treatments and therefore were not sufficient to compensate for the growth slowdown in the first part of development. The presence of a predator in late tadpole development influenced neither the time to metamorphosis nor size/body mass at metamorphosis. In conclusion, the predator had the effect on metamorphosis traits only if it was present in the early development of tadpoles.

• MeSH
• biologická proměna MeSH
• larva MeSH
• predátorské chování * MeSH
• Rana temporaria MeSH
• žáby MeSH
• želvy * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: With advancements in diagnostic techniques, oligometastatic prostate cancer is diagnosed in patients who were, in the past, considered to have localized disease. Moreover, evidence of the effectiveness of treatment intensification for this disease is increasing, focusing on primary tumors as well as metastatic lesions. Thus, we can delay the start of systemic palliative treatment and improve overall survival. Many questions remain unclear, such as the definition of oligometastasis disease, or which patients should be offered aggressive treatment. Data are limited and come from small retrospective studies but show conclusively the benefits of survival in targeted primary prostate and metastatic prostate cancer therapy with surgery or radiotherapy. Often, stereotactic radiotherapy is used in this indication, with minimal side effects. In retrospective studies, 3-5 metastatic lesions were generally accepted for definition of oligometastatic disease, but patient subgroups were heterogeneous. A recent study attempts to better define oligometastatic disease and find out the right degree of intensification of treatment. When and in which patient to use metastasis-targeted therapy and when the standard systemic treatment is already meaningful. It is already clear that selected patients benefit from targeted personalized treatment. PURPOSE: The purpose of this review is to offer an update of the problem of oligometastatic prostate cancer. The article presents an overview of data from contemporary literature, modern possibilities of diagnostic imaging methods and treatment options of oligometastatic prostate cancer including surgery and radiotherapy. authors declare they have no potential conflicts of interest concerning drugs, products, or services used in the study. The Editorial Board declares that the manuscript met the ICMJE recommendation for biomedical papers. Submitted: 8. 2. 2019 Accepted: 5. 3. 2019.

• Klíčová slova
• PET/CT, androgen-deprivation therapy, oligometastatic disease, prostate cancer, prostatectomy, radiotherapy,
• MeSH
• lidé MeSH
• metastázy nádorů MeSH
• nádory prostaty patologie   terapie   MeSH
• prognóza MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

BACKGROUND: In the Czech Republic, around 6,500 women get breast cancer each year; out of this number, nearly 1,000 women are triple negative subtype. Triple negative breast cancer is characterized by lack of expression of α-estrogen, progesterone, and HER2 receptors. Vast majority of these cases are low-differentiated carcinomas, majority belonging to the basal-like subgroup defined originally by DNA chips. Clinically, they are characterized by greater aggressiveness, frequent rate of local recurrence and organ metastases. They are more common in younger women and are associated with the occurrence of hereditary forms of breast cancer caused by pathogenic mutations in the BRCA1 gene and in rare cases also BRCA2. AIM: The objective of this review is to provide comprehensive information about current knowledge of triple negative breast cancer. This paper summarizes information about epidemiology and etiopathogenesis of this disease, describes risk factors for both sporadic and hereditary forms of triple negative breast cancer, addresses histopathologic and molecular classification of triple negative breast cancer, and these characteristics associates with treatment and prediction of disease development. The article also addresses new anticancer drugs tested for triple negative breast cancer. CONCLUSION: Triple negative breast cancer is a heterogeneous group of diseases with limited therapeutic options. The key to further shift in therapy is detailed knowledge of its clinical and molecular diversity and identification of predictive biomarkers. Further improvement of therapy results of triple negative breast cancer cannot be expected before targeted therapy of this disease is found.

• MeSH
• lidé MeSH
• triple-negativní karcinom prsu epidemiologie   patologie   terapie   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH

The immune system is believed to play a dual role in carcinogenesis. On one hand, it could prompt tumorigenesis and cancer progression, on the other hand, it has the capacity to eradicate tumor cells. There has been an evidence of natural immunogenicity in breast cancer and we have also witnessed several attempts to stimulate non-specific antitumor immune response (Coleys toxin, BCG vaccine etc.). New technologies and further knowledge of molecular basis of immune system and its function encouraged the development of effective immunotherapy capable of inducing a solid antitumor activity. These agents appear promissing in the prevention and therapy of breast carcinoma as well. The assumption is based on the results of several antitumor vaccine trials targeted against HER2, MUC1, CEA and mammaglobin-A, as well as immune checkpoint inhibitors (e.g. CTLA-4, PD-1/ PD-L1, LAG3). With regards to different mech-anisms of action of these agents, their combination might bring about synergistic antitumor effects. Nonetheless, monoclonal antibodies and cytostatic agents already approved for breast cancer treatment might be exploited for their immunomodulation effect as well. This article addresses prospects for immunotherapy of breast carcinoma in detail.

• MeSH
• imunoterapie metody   MeSH
• lidé MeSH
• nádory prsu imunologie   prevence a kontrola   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

Cyclin-dependent kinases (CDKs) participate in many cellular processes and play a crucial role in the regulation of cell cycle and transcription processes. Recently, CDK12 was identified as a key factor orchestrating transcription of genes, such as BRCA1, ATM, ATR, FANCI and FANCD2, which are involved in the DNA-damage response pathway. Importantly, inhibition of function of these genes commonly leads to induction of genomic instability followed by cancer development, but the precise contribution of CDK12 to these processes is to be unveiled. Nevertheless, several mutations affecting function of CDK12 were already identified in a variety of tumors of different origin (ovary, breast, prostate, intestine) making tumors sensitive to cytostatics promot-ing DNA damage (platin derivatives, alkylating regimens) and inhibitors of DNA repair (PARP inhibitors). Such an effect has been already observed in the model of high grade serous ovarian carcinomas. Thus, CDK12 is becoming a potential therapeutic target of drugs causing synthetic lethality in these cells. Our review summarizes most recent information about CDK12 function in cancer and discusses potential use of CDK12 in clinics.

• MeSH
• alkylační protinádorové látky terapeutické užití   MeSH
• chromozomální nestabilita MeSH
• cisplatina terapeutické užití   MeSH
• cyklin-dependentní kinasy genetika   fyziologie   MeSH
• genetická transkripce * MeSH
• karcinogeneze * genetika   MeSH
• lidé MeSH
• mutace MeSH
• nádory vaječníků farmakoterapie   MeSH
• nádory farmakoterapie   genetika   MeSH
• oprava DNA * MeSH
• poškození DNA * MeSH
• protinádorové látky terapeutické užití   MeSH
• Check Tag
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• alkylační protinádorové látky MeSH
• CDK12 protein, human MeSH Prohlížeč
• cisplatina MeSH
• cyklin-dependentní kinasy MeSH
• protinádorové látky MeSH

CASE: Herein we report a case of a man with a B- cell non-Hodgkin lymfoma, primarily diagnosed by topographic and morfology tokens as lobular breast carcinoma and, as such, it was treated by chemotherapy and endocrine therapy. The treatment resulted in complete remission for 3,5 years. However, the subsequent relapses that arised in retrocrural and left axilary area did not respond adequately to breast cancer targeted chemotherapy. Therefore the patient underwent re-exstirpation of axillary lymph node yielding a surprising histology finding of folicular lymphoma. The primary biopsy specimen was histologicaly reevaluated and the initial dia-gnosis was reclassified as folicular lymphoma. The patient was given an adequate chemotherapy and targeted treatment that established a complete remission. Six months afterwards there was a relapse detected in the retrocrural area. The patient underwent palliative radiotherapy that brought about complete remission and, so far, he is in good condition. It has been eight years since the cancer dia-gnosis was established. This case report is appended by review of literature dealing with diagnostic confusion of these two malignancies. CONCLUSION: Re -biopsy plays a significant role in case of treatment strategy controversies, predominantly on condition of atypical course of malignant disease. It should always be considered in case of cancer relapse, especially if the phenotype specfication could affect the treatment decision.

• MeSH
• B-buněčný lymfom patologie   MeSH
• biopsie MeSH
• chybná diagnóza * MeSH
• folikulární lymfom patologie   MeSH
• lidé MeSH
• lobulární karcinom patologie   MeSH
• lokální recidiva nádoru patologie   MeSH
• nádory prsu u mužů patologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• kazuistiky MeSH
• přehledy MeSH

This study aimed to determine whether maternal cell contamination exists in cells derived from equine umbilical cord tissue, a perspective material for cell-based therapies in veterinary medicine. Potential maternal cell contamination was analyzed at DNA level via a set of 16 microsatellite markers in cells originating from the cord tissue of 22 foals. In these cells no maternal cell contamination was detected at a sensitivity level of 0.01%. Our results suggest that equine umbilical cord tissue-derived cells are entirely of fetal origin.

• Klíčová slova
• Maternal cell contamination, Mesenchymal stromal cells, Microsatellite markers, Umbilical cord tissue,
• MeSH
• koně * MeSH
• kultivované buňky * MeSH
• mezenchymální kmenové buňky * MeSH
• mikrosatelitní repetice MeSH
• pupečník cytologie   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH