We aimed to perform a chemical analysis of both Alibernet red wine and an alcohol-free Alibernet red wine extract (AWE) and to investigate the effects of AWE on nitric oxide and reactive oxygen species production as well as blood pressure development in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHRs). Total antioxidant capacity together with total phenolic and selected mineral content was measured in wine and AWE. Young 6-week-old male WKY and SHR were treated with AWE (24,2 mg/kg/day) for 3 weeks. Total NOS and SOD activities, eNOS and SOD1 protein expressions, and superoxide production were determined in the tissues. Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine. The AWE increased NOS activity in the left ventricle, aorta, and kidney of SHR, while it did not change NOS activity in WKY rats. Similarly, increased SOD activity in the plasma and left ventricle was observed in SHR only. There were no changes in eNOS and SOD1 expressions. In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR. Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.
- MeSH
- antioxidancia chemie farmakologie MeSH
- aorta účinky léků enzymologie MeSH
- hypertenze metabolismus patologie MeSH
- krysa rodu Rattus MeSH
- ledviny účinky léků enzymologie MeSH
- minerály analýza farmakologie MeSH
- oxid dusnatý metabolismus MeSH
- polyfenoly analýza farmakologie MeSH
- potkani inbrední SHR MeSH
- potkani inbrední WKY MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rostlinné extrakty chemie farmakologie MeSH
- srdeční komory účinky léků enzymologie MeSH
- superoxiddismutasa 1 MeSH
- superoxiddismutasa metabolismus MeSH
- synthasa oxidu dusnatého, typ III metabolismus MeSH
- víno analýza MeSH
- Vitis chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- minerály MeSH
- oxid dusnatý MeSH
- polyfenoly MeSH
- reaktivní formy kyslíku MeSH
- rostlinné extrakty MeSH
- Sod1 protein, rat MeSH Prohlížeč
- superoxiddismutasa 1 MeSH
- superoxiddismutasa MeSH
- synthasa oxidu dusnatého, typ III MeSH
