PREMISE OF THE STUDY: The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. METHODS AND RESULTS: Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with public DNA sequence databases. Interspecific variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for other Asteraceae tribes. All three markers had higher variation (2.1-4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplification was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. CONCLUSIONS: The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, especially for very closely related species.

• MeSH
• Asteraceae klasifikace   genetika   MeSH
• buněčné jádro genetika   MeSH
• chromozomy rostlin genetika   MeSH
• diploidie MeSH
• DNA primery genetika   MeSH
• DNA rostlinná genetika   MeSH
• fylogeneze MeSH
• genetická variace MeSH
• genetické markery MeSH
• genová dávka MeSH
• genová knihovna MeSH
• intergenová DNA genetika   MeSH
• pseudogeny MeSH
• rostlinné geny * MeSH
• sekvence nukleotidů MeSH
• sekvenční analýza DNA MeSH
• výpočetní biologie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA primery MeSH
• DNA rostlinná MeSH
• genetické markery MeSH
• intergenová DNA MeSH