A novel HLA-B (human leukocyte antigen-B) allele, HLA-B*4442, was identified both in a Czech patient with leukaemia and in his mother. The presence of a novel allele was initially suspected because conflicting results were obtained by serological and DNA typing techniques. The HLA typing using the polymerase chain reaction-sequence-specific primers (PCR-SSP) at the two-digit level indicated an allele belonging to the HLA-B*44 group, whereas serological typing indicated HLA-B21. Typing with PCR-sequence-specific oligonucleotides (PCR-SSO) resulted in a unique reaction pattern that could not be assigned to a known allele, PCR-SSP typing at the four-digit level did not match any known B*44 allele, either. The sequencing-based typing of the HLA-B locus then revealed the novel B*4442 allele that is identical with B*4405 except a single C-->G nucleotide exchange at position 572. This exchange results in an amino acid substitution from serine to tryptophan at position 167 of the expressed HLA-B protein. The B21 serological reactivity of the novel B*4442 allele product was confirmed by employing an additional serological panel of typing sera. Our findings support previous reports claiming that serine at the position 167 in the alpha-2 domain of the HLA-B protein is a major determinant of the HLA-B44(12) serological epitope.
- MeSH
- alely * MeSH
- bodová mutace * imunologie MeSH
- HLA-B antigeny genetika imunologie MeSH
- HLA-B44 antigen MeSH
- leukemie genetika imunologie MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- regulace genové exprese u leukemie genetika imunologie MeSH
- rodokmen MeSH
- sekvence nukleotidů MeSH
- substituce aminokyselin * imunologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- HLA-B antigeny MeSH
- HLA-B*44:42 antigen MeSH Prohlížeč
- HLA-B21 antigen MeSH Prohlížeč
- HLA-B44 antigen MeSH
