Fructobacillus, a Gram-positive, non-spore-forming, facultative anaerobic bacterium, belongs to the fructophilic lactic acid bacteria (FLAB) group. The group's name originates from fructose, the favored carbon source for its members. Fructobacillus spp. are noteworthy for their distinctive traits, captivating the interest of scientists. However, there have been relatively few publications regarding the isolation and potential utilization of these microorganisms in the industry. In recent years, F. tropaeoli has garnered interest for its promising role in the food and pharmaceutical sectors, although the availability of isolates is rather limited. A more comprehensive understanding of Fructobacillus is imperative to evaluate their functionality in the industry, given their unique and exceptional properties. Our in vitro study on Fructobacillus tropaeoli KKP 3032 confirmed its fructophilic nature and high osmotolerance. This strain thrives in a 30% sugar concentration, shows resistance to low pH and bile salts, and exhibits robust autoaggregation. Additionally, it displays significant antimicrobial activity against foodborne pathogens. Evaluating its probiotic potential, it aligns with EFSA recommendations in antibiotic resistance, except for kanamycin, to which it is resistant. Further research is necessary, but preliminary analyses confirm the high probiotic potential of F. tropaeoli KKP 3032 and its ability to thrive in the presence of high concentrations of fructose. The results indicate that the isolate F. tropaeoli KKP 3032 could potentially be used in the future as a fructophilic probiotic, protective culture, and/or active ingredient in fructose-rich food.

• Klíčová slova
• Antimicrobial activity, Autoaggregation, Bile salts, FLAB, Fructophilic probiotic, Hydrophobicity,
• MeSH
• antibakteriální látky farmakologie   MeSH
• fruktosa metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• ovocné a zeleninové šťávy * mikrobiologie   MeSH
• pomerančovník čínský mikrobiologie   chemie   MeSH
• potravinářská mikrobiologie MeSH
• probiotika * izolace a purifikace   MeSH
• RNA ribozomální 16S genetika   MeSH
• žlučové kyseliny a soli metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• fruktosa MeSH
• RNA ribozomální 16S MeSH
• žlučové kyseliny a soli MeSH

Co-pyrolysis of orange peel and chicken eggshell was performed for the synthesis of the composite, a co-pyrolysis technique used to promote natural fabrication and to allow the raw material elemental combination effect and the preparatory conditions such as pyrolysis temperature, residence time, and eggshell/orange peel mixing ratio, to be optimized with the response surface methodology through Box-Behnken Design(BBD). BBD involved a randomized series of 17 experimental runs, and the best optimal conditions were found with a pyrolysis temperature of 300 °C, a residence time of 1 h, and 0.5 as the mixing ratio. These conditions gave a maximum adsorption capacity of 167 mg/g for removal of the modal pollutant methylene blue. FTIR spectra of the composite showed new functional peaks of oxygenated groups, at two different bands. XRD confirmed an amorphous surface with inorganic component peaks, while SEM-EDS revealed rich defects sites along with an enhanced percentage of oxygen elements on the surface; the surface area was enhanced from 1 m2 with unmodified peel to 64 m2 with composite. The adsorption behavior of the composite was studied for dye removal and the adsorption behavior was well explained by the Langmuir isotherm model.

• Klíčová slova
• Adsorption, Co-pyrolysis, Methylene blue, Optimization, Orange peel & eggshell composite,
• MeSH
• adsorpce MeSH
• chemické látky znečišťující vodu * MeSH
• kinetika MeSH
• methylenová modř MeSH
• pomerančovník čínský * MeSH
• pyrolýza MeSH
• teplota MeSH
• vaječná skořápka MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chemické látky znečišťující vodu * MeSH
• methylenová modř MeSH

Vitamin C deficiency resulting in scurvy, is considered to be a rare nutritional disorder in developed countries, thus leading to underdiagnosis with exposure to unnecessary investigations and delay in appropriate treatment. The wide myriad of clinical signs and symptoms with which vitamin C deficiency can present (including haematological, musculoskeletal and vague constitutional symptoms that overlap with other common medical conditions), also contributes to this diagnostic challenge. Despite scurvy being habitually thought to be present in children with neurodevelopmental conditions such as autism spectrum disorder, other important at-risk groups that frequently tend to be forgotten include children with persistent fussy eating behaviour, and children with abnormal vitamin C metabolism. We hereunder present a case of a 10-year-old boy who presented to an acute general hospital for further investigation with gait disturbance. The lack of detailed nutritional assessment on presentation in the first instance led to a missed diagnosis of vitamin C deficiency, thus exposing the child to a wide array of unnecessary investigations and treatments. The added perplexity to the case resulting from false positive results of investigations performed as part of this child's workup, is also discussed.

• Klíčová slova
• Scurvy, ascorbic acid deficiency, case report, child nutrition disorder, nutrition assessment, nutritional deficiency, pediatrics,
• MeSH
• chůze (způsob) MeSH
• dítě MeSH
• kurděje * komplikace   diagnóza   farmakoterapie   MeSH
• kyselina askorbová terapeutické užití   MeSH
• lidé MeSH
• nedostatek vitaminu C * komplikace   diagnóza   farmakoterapie   MeSH
• pomerančovník čínský * MeSH
• poruchy autistického spektra * komplikace   diagnóza   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Názvy látek
• kyselina askorbová MeSH

Mitidjospirone, a new spiridioxynaphthalene, was isolated from the mycelial extract of a strain of Lasiodiplodia mitidjana, a recently described species belonging to the family Botryosphaeriaceae. Its structure was elucidated by extensive spectroscopic analysis and the absolute configuration was determined by electronic circular dichroism (ECD) experiment. Furthermore, several known compounds were identified during the screening of secondary metabolites produced by four strains of L. mitidjana.

• Klíčová slova
• Botryosphaeriaceae, GC-MS, fungal metabolites, metabolomics, natural products,
• MeSH
• Ascomycota * metabolismus   MeSH
• fylogeneze MeSH
• mitosporické houby * MeSH
• nemoci rostlin MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
• pomerančovník čínský * MeSH
• Publikační typ
• časopisecké články MeSH

Several Botryosphaeriaceae species are known to occur worldwide, causing dieback, canker and fruit rot on various hosts. Surveys conducted in ten commercial citrus orchards in the northern region of Algeria revealed five species of Botryosphaeriaceae belonging to three genera associated with diseased trees. Morphological and cultural characteristics as well as phylogenetic analyses of the internal transcribed spacer (ITS) region and the translation elongation factor 1-alpha (tef1-α) identified Diplodia mutila, Diplodia seriata, Dothiorella viticola, Lasiodiplodia mediterranea and a novel species which is here described as Lasiodiplodia mithidjana sp. nov.. Of these, L. mithidjana (14.1% of the samples) and L. mediterranea (13% of the samples) were the most widespread and abundant species. Pathogenicity tests revealed that L. mediterranea and D. seriata were the most aggressive species on citrus shoots. This study highlights the importance of Botryosphaeriaceae species as agents of canker and dieback of citrus trees in Algeria.

• MeSH
• Ascomycota klasifikace   genetika   patogenita   MeSH
• DNA fungální genetika   MeSH
• druhová specificita MeSH
• fylogeneze MeSH
• nemoci rostlin mikrobiologie   MeSH
• pomerančovník čínský mikrobiologie   MeSH
• virulence MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Alžírsko MeSH
• Názvy látek
• DNA fungální MeSH

In this contribution we present an innovative way to easy, fast, and highly sensitive analyses by CE with ESI-MS detection. The new method is designed to be applied to ESI-compatible electrolytes (e.g. ammonium acetate) and offers advanced tuning of selectivity conditions within a wide range of analyte mobilities. We use a full capillary ITP format to provide powerful on-line analyte stacking at the ITP boundary all the way to detection and introduce the model of extended ITP where a controlled concentration of the leading ion is added to the terminating zone. Such systems preserve all properties of an ITP system and the velocity of the stacking ITP boundary can be tuned by the composition of both the leading and terminating zone. In this way, the system properties can be controlled flexibly and the mobility window of stacked analytes can be tailored to actual needs. The presented theory and the newly defined concept of zone-related boundary mobility allow easy assessment of system selectivity using simple diagrams. We demonstrate the model and its potential on the example of simple acidic cationic systems composed of only two substances (ammonium and acetate) including the example of thiabendazole analysis with a detection limit of 10(-10) M (20 ng/L) and its determination in orange juice by direct sampling after filtration, selective stacking by a tuned extended ITP system, and ESI-MS detection.

• MeSH
• acetáty chemie   MeSH
• elektroforéza kapilární metody   MeSH
• elektrolyty chemie   MeSH
• hmotnostní spektrometrie s elektrosprejovou ionizací metody   MeSH
• izotachoforéza metody   MeSH
• kationty analýza   chemie   MeSH
• limita detekce MeSH
• nápoje analýza   MeSH
• pomerančovník čínský chemie   MeSH
• rezidua pesticidů analýza   chemie   MeSH
• thiabendazol analýza   chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acetáty MeSH
• ammonium acetate MeSH Prohlížeč
• elektrolyty MeSH
• kationty MeSH
• rezidua pesticidů MeSH
• thiabendazol MeSH